Beta-NGF 蛋白, Human (HEK293)

Nerve Growth Factor-β (Beta-NGF; NGF) is a neurotrophic factor that regulates neuronal survival and differentiation. NGF is also a seminal plasma protein involved in ovulation and luteinizing. NGF has potential functions in the female reproductive system to help overcome the current problem of early embryo loss^[1][2][3]. Beta-NGF Protein, Human (HEK293) is produced by HEK293 cells (S122-R239), with tag free.

神经生长因子-β（Beta-NGF；NGF）是一种由 118 个氨基酸组成的碱性蛋白，作为周围神经系统的感觉和交感神经元以及前基底大脑的胆碱能神经元的营养因子^[1]。NGF 参与神经元存活和分化的调节。NGF 水平升高与创伤后应激障碍 (PTSD) 的风险相关。创伤反应导致负责 NGF 表达的 DNA 核苷酸甲基化。NGF 水平显示交感纤维密度的增加与 NGF 信使 RNA (mRNA) 水平成正比。NGF也是哺乳动物中常见的精浆蛋白。例如，NGF 作为骆驼的排卵刺激因子，并已被证明对公牛具有促黄体生成作用。NGF 在女性生殖系统中具有潜在的功能。例如，NGF在排卵诱导、LH释放、排卵、黄体发育、黄体酮（P4）产生、黄体体血管化和促性腺激素反应中发挥重要作用。对牛施用 NGF 可通过增加 LH 释放来增强类固醇产生、黄体形成和功能，并导致下游妊娠和发育标志物 mRNA 表达增加。此外，NGF 潜在的黄体生成作用有助于克服目前早期胚胎丢失的问题^[2][3]。人和牛NGF蛋白序列相似度为90.87%。同时，人NGF与大鼠和小鼠的相似性分别为85.89%和85.06%。

NGF (重组人源蛋白; 100 ng/mL; 5 d) 诱导 B 细胞的 DNA 合成呈剂量依赖性增加^[4]。

NGF (重组人源蛋白; 100 ng/mL; 5 d) 诱导 B 细胞的 DNA 合成呈剂量依赖性增加^[4]。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is ≤2 ng/mL.

• 
  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1.104 ng/mL, corresponding to a specific activity is 9.06×10^5 units/mg.

Human

HEK293

P01138 (S122-R239)

4803  [NCBI]

SSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVR

Approximately 13.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM PB, 250 mM NaCl, pH7.0 or 20 mM NaAC, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yue XJ, et al. Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression. PLoS One. 2013;8(4):e62024. Published 2013 Apr 24.  [Content Brief]

  [2]. Lipov EG, et al. Possible Reversal of PTSD-Related DNA Methylation by Sympathetic Blockade. J Mol Neurosci. 2017 May;62(1):67-72.  [Content Brief]

  [3]. Castellanos MR, et al. Obtención y caracterización del beta-NGF murino. Aplicación en un modelo de envejecimiento cerebral [Obtention and characterization of murine beta-NGF. Application in a model of cerebral aging]. Rev Neurol. 1998;26(153):717-722.  [Content Brief]

  [4]. Lima FS, et al. Insights into nerve growth factor-β role in bovine reproduction - Review. Theriogenology. 2020 Jul 1;150:288-293.  [Content Brief]

  [5]. Otten U, et al. Nerve growth factor induces growth and differentiation of human B lymphocytes. Proc Natl Acad Sci U S A. 1989 Dec;86(24):10059-63.  [Content Brief]