Beta-NGF 蛋白, Mouse (110a.a, His)

Beta-NGF protein is essential for the development and maintenance of the nervous system and binds to NTRK1 and NGFR receptors to initiate signaling cascades that regulate neuronal processes.The NGF precursor proNGF acts as a ligand for the SORCS2-NGFR receptor and activates pathways affecting RAC1/2, the actin cytoskeleton, and neuronal growth.Beta-NGF Protein, Mouse (110a.a, His) is the recombinant mouse-derived Beta-NGF protein, expressed by E.coli , with N-6*His labeled tag.

Beta-NGF 是交感神经和感觉神经系统发育和维持的关键因子，作为 NTRK1 和 NGFR 受体的细胞外配体，触发调节神经元增殖、分化和存活的细胞信号级联。未成熟的 NGF 前体 (proNGF) 作为 SORCS2 和 NGFR 形成的异二聚体受体的配体，诱导信号级联反应，导致 RAC1 和/或 RAC2 失活，以及肌动蛋白细胞骨架的重组和随后的神经元生长锥崩溃。与成熟的 NGF 相比，proNGF 已被鉴定可在体外促进神经元凋亡。此外，Beta-NGF 还可抑制金属蛋白酶依赖性血小板糖蛋白 VI 的蛋白水解作用。Beta-NGF 在其同二聚体结构中与溶血磷脂酰肌醇和溶血磷脂酰丝氨酸结合，在细胞反应中表现出多种功能，包括在脂质结合形式下促进肥大细胞释放组胺。Beta-NGF 的同二聚体结构与 NTRK1、NGFR 和 SORCS2 相互作用，而 NGF 前体 (proNGF) 与 SORT1 和 NGFR 形成的受体复合物结合，导致 NGF 内吞作用。这些复杂的相互作用强调了 Beta-NGF 在协调神经元功能和细胞反应中的多方面作用。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 9.125 ng/mL, corresponding to a specific activity is 1.095×10^5 units/mg.

Mouse

E. coli

N-6*His

P01139 (M130-R239)

18049

MGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATR

Approximately 12.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.