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  3. Blot21 蛋白, Blomia tropicalis (HEK293, His)

Blot21 蛋白, Blomia tropicalis (HEK293, His)

目录号: HY-P77307
产品使用指南

Blot21 蛋白是一种重要的热带 Blomia 过敏原,是一组新的过敏原。热带 Blomia 的过敏成分是热带地区过敏的重要诱因。Blot21 是第 5 组过敏原 Blot5 的旁系同源物。Blot21 与 Blot5 具有中等序列同一性 (40.7%),与 Blot5 具有低至中度交叉反应性。Blot21 蛋白, Blomia tropicalis (HEK293, His) 是重组的 Blot21 蛋白,由 HEK293 表达,带有 N-6*His 标签。

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描述

Blot21 Protein, representing a new group of allergens, is an important Blomia tropicalis allergen. Allergenic components from Blomia tropicalis are important triggers of allergies in the tropics. Blot21 is a paralog of the group 5 allergen Blot5. Blot21 has moderate sequence identity (40.7%) to Blot5 and low to moderate cross-reactivity to Blot5. Blot21 Protein, Blomia tropicalis (HEK293, His) is the recombinant Blot21 protein, expressed by HEK293 , with N-6*His labeled tag.

研究背景

Blot21 是一种重要的热带 Blomia 过敏原,是一组新的过敏原。热带 Blomia 的过敏成分是热带地区过敏的重要诱因。Blot21 是第 5 组过敏原 Blot5 的旁系同源物。Blot21 与 Blot5 具有中等序列同一性 (40.7%),与 Blot5 具有低至中度交叉反应性。Blot21 由三个反平行 α 螺旋组成,组装成螺旋束,类似于 Blot5 和 Derp5[1][2]

种属

Others

表达系统

HEK293

标签

N-6*His

蛋白编号

AAX34047 (L17-E129)

基因 ID

/

蛋白结构
N-term
6*His
Blot21 (L17-E129)
Accession # AAX34047
C-term
同用名
Mite allergen Blo t 21; Mite group 21 allergen Blo t 21; Blo t 21
分子量

Approximately 16.3 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Blot21 Protein, Blomia tropicalis (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Blot21 Protein, Blomia tropicalis (HEK293, His)
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HY-P77307
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