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  3. Blot21 蛋白, Blomia tropicalis (His)

Blot21 蛋白, Blomia tropicalis (His)

目录号: HY-P77306
COA 产品使用指南

Blot21 蛋白是一种重要的热带 Blomia 过敏原,是一组新的过敏原。热带 Blomia 的过敏成分是热带地区过敏的重要诱因。Blot21 是第 5 组过敏原 Blot5 的旁系同源物。Blot21 与 Blot5 具有中等序列同一性 (40.7%),与 Blot5 具有低至中度交叉反应性。Blot21 蛋白, Blomia tropicalis (His) 是重组的 Blot21 蛋白,由 E. coli 表达,带有 N-6*His 标签。Blot21 蛋白, Blomia tropicalis (His) 全长 113 个氨基酸,分子量约为 ~13.5 kDa。

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5 μg ¥600 In-stock
10 μg ¥1000 In-stock
50 μg ¥2800 In-stock
100 μg ¥4750 In-stock
500 μg ¥13500 询价
1 mg ¥23000 In-stock
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描述

Blot21 Protein, representing a new group of allergens, is an important Blomia tropicalis allergen. Allergenic components from Blomia tropicalis are important triggers of allergies in the tropics. Blot21 is a paralog of the group 5 allergen Blot5. Blot21 has moderate sequence identity (40.7%) to Blot5 and low to moderate cross-reactivity to Blot5. Blot21 Protein, Blomia tropicalis (His) is the recombinant Blot21 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Blot21 Protein, Blomia tropicalis (His) is 113 a.a., with molecular weight of ~13.5 kDa.

研究背景

Blot21 是一种重要的热带 Blomia 过敏原,是一组新的过敏原。热带 Blomia 的过敏成分是热带地区过敏的重要诱因。Blot21 是第 5 组过敏原 Blot5 的旁系同源物。Blot21 与 Blot5 具有中等序列同一性 (40.7%),与 Blot5 具有低至中度交叉反应性。Blot21 由三个反平行 α 螺旋组成,组装成螺旋束,类似于 Blot5 和 Derp5[1][2]

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

AAX34047.1 (L17-E129)

基因 ID

/

蛋白结构
N-term
6*His
Blot21 (L17-E129)
Accession # AAX34047.1
C-term
同用名
Mite allergen Blo t 21; Mite group 21 allergen Blo t 21; Blo t 21
氨基酸序列

LPVSNDNFRHEFDHMIVNTATQRFHEIEKFLLHITHEVDDLEKTGNKDEKARLLRELTVSEAFIEGSRGYFQRELKRTDLDLLEKFNFEAALATGDLLLKDLKALQKRVQDSE

分子量

Approximately 13.5 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Blot21 Protein, Blomia tropicalis (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Blot21 Protein, Blomia tropicalis (His)
目录号:
HY-P77306
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