BMI1 蛋白, Human (His)

BMI1 Protein, a component of the Polycomb group PRC1-like complex, maintains transcriptional repression in genes, including Hox genes, by monoubiquitinating histone H2A. It regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2. Interacts with various partners in the PRC1-like complex, such as RNF2, CBX2, CBX4, CBX8, PHC1, PHC2, PHC3, and UB2D3, forming complexes crucial for chromatin modification and gene expression control. BMI1 Protein, Human (E.coli,N-His) is the recombinant human-derived BMI1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of BMI1 Protein, Human (E.coli,N-His) is 326 a.a., with molecular weight of ~43 kDa.

BMI1 蛋白是 Polycomb 族 (PcG) 多蛋白 PRC1 样复合物的关键组成部分，对于在整个发育过程中维持包括 Hox 基因在内的各种基因的转录抑制状态至关重要。这种 PcG PRC1 复合物通过染色质重塑和组蛋白修饰发挥作用，特别介导组蛋白 H2A 在“Lys-119”处的单泛素化，从而赋予染色质表达性的可遗传变化。在 PRC1 样复合物中，BMI1 调节 RNF2/RING2 的 E3 泛素蛋白连接酶活性。 BMI1 与 RING1、CBX7、CBX8 和 PHC2 等各种伙伴相互作用，在核小体结合和组蛋白 H2A 修饰中发挥着关键作用。此外，BMI1 形成包含 CUL3 和 SPOP 的更大复合体的一部分，并与 E4F1 和锌指蛋白 ZNF277 相互作用，表明它参与了复杂的蛋白质网络，有助于发育过程中的染色质动力学和基因调控。

Human

E. coli

N-6*His

P35226 (M1-G326)

648

MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG

Approximately 43 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.