BMP-13/GDF-6 蛋白, Mouse

The BMP-13/GDF-6 protein is a multifunctional growth factor that plays a critical role in retinal development, control of apoptosis, and dorsoventral positional information. It is critical for bone and joint development in various anatomical regions, influencing species-specific skeletal changes and contributing to the evolution of unique anatomical features. BMP-13/GDF-6 Protein, Mouse is the recombinant mouse-derived BMP-13/GDF-6 protein, expressed by E. coli , with tag free. The total length of BMP-13/GDF-6 Protein, Mouse is 120 a.a., with molecular weight of ~13.7 kDa.

BMP-13/GDF-6 蛋白是一种多功能生长因子，在细胞过程中发挥关键作用，包括调节视网膜和骨形成中的增殖和分化。它在视网膜发育中的关键参与包括细胞凋亡的控制和背腹位置信息的建立，这对于视网膜顶盖图的形成至关重要。而且，BMP-13/GDF-6对于四肢、头骨、手指、中轴骨骼等各个解剖区域的骨关节的正常发育是不可或缺的。它的重要性延伸到塑造物种特异性的骨骼结构变化，表明在不同解剖特征的进化中发挥作用。这种多方面的生长因子通过特定受体亚基（包括 BMPR1A、BMPR1B、BMPR2 和 ACVR2A）积极调节软骨形成组织分化，激活 SMAD1-SMAD5-SMAD8 复合物。值得注意的是，软骨形成分化的调节面临 NOG 的抑制。此外，BMP-13/GDF-6 利用生长因子受体亚基 BMPR1A、BMPR2 和 ACVR2A 参与诱导间充质干细胞的脂肪生成，同时激活 SMAD1-SMAD5-SMAD8 复合物和 MAPK14/p38。这种复杂的分子芭蕾通过同二聚化展开，形成二硫键连接的结构。

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is typically 0.85-5 µg/mL.

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  Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ this effect is 1.808 μg/mL, corresponding to a specific activity is 553.097 units/mg.

Mouse

E. coli

Tag Free

P43028 (T335-R454)

242316  [NCBI]

TAFASRHGKRHGKKSRLRCSRKPLHVNFKELGWDDWIIAPLEYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPGSTPPSCCVPTKLTPISILYIDAGNNVVYKQYEDMVVESCGCR

Approximately 13.7 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from sterile 50 mM Tris-HCL, 300 mM NaCL, 500 mM arginine, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 300 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.