1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Bone Morphogenetic Proteins (BMPs)
  5. Bone Morphogenetic Protein 2
  6. BMP-2 蛋白, Human/Mouse/Rat

BMP-2 蛋白, Human/Mouse/Rat

目录号: HY-P7006
COA 产品使用指南

骨形态发生蛋白2 (BMP-2) 是一种多效性配体蛋白,属于 TNFβ 家族,并参与关键胚胎发育的血管和瓣膜稳态。BMP-2 结合 I 型受体 (ALK-2/-3/-6) 和 II 型受体 (BMPR2, ACVR2A),以调节各种钙化类型,包括动脉粥样硬化、慢性肾病、糖尿病、瓣膜钙化。BMP-2 在人钙化瓣膜的骨化区域中由 B 淋巴细胞和 T 淋巴细胞密集浸润区域的肌成纤维细胞和前成骨细胞过度表达,是动脉粥样硬化血管钙化与正常骨形成机制之间的连接点。重组人/小鼠/大鼠 BMP-2 蛋白长 114 个氨基酸 (Q283-R396),由大肠杆菌表达。

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规格 价格 是否有货 数量
10 μg ¥1400 In-stock
50 μg ¥4600 In-stock
100 μg ¥7820 询价
> 100 μg   询价  

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BMP-2 Protein, Human/Mouse/Rat 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Bone morphogenetic protein 2 (BMP-2) is a pleiotropic ligand protein belonging to TNFβ family, and is involved in key embryonic development of vascular and valvular homeostasis. BMP-2 binds to type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A) to regulate various types of calcification, including atherosclerosis, chronic kidney disease, diabetes, and valve calcification[1]. BMP-2 is overexpressed by myofibroblast and preosteoblast in the calcified area of human calcified valve, which are densely infiltrated by B lymphocytes and T lymphocytes[2]. BMP-2 is the junction between atherosclerotic vascular calcification and normal bone formation mechanism[3]. BMP-2 Protein, Human/Mouse/Rat is 114 a.a. (Q283-R396), expressed in E. coli.

研究背景

骨形态发生蛋白2(BMP-2)是一种多效性配体蛋白,属于TNFβ家族。BMP-2 格式化 BMP/TGFβ 信号传导以参与血管和瓣膜稳态,这是胚胎发育的关键过程[1]
BMP-2/TGFβ 信号传导可通过抑制性终止SMAD 包括 SMAD6 和 SMAD7,它们由 BMP 信号传导激活和诱导,并通过多种机制关闭 BMP 信号传导[4]
BMP-2 广泛存在于不同动物中,而其序列人体中的情况与大鼠 (91.86%) 和小鼠 (92.13%) 相似。
BMP 对动脉粥样硬化、肺血管疾病以及血管和瓣膜钙化的病理生理学具有重要贡献[1]
BMP-2结合不同的受体,如I型受体(ALK-2/-3/-6)和II型受体(BMPR2、ACVR2A),调节多种钙化类型,包括动脉粥样硬化、慢性肾脏病、糖尿病、瓣膜钙化[1]
BMP-2 促进单核细胞浸润和动脉粥样硬化军团的炎症[5]
它与通过促炎和促动脉粥样硬化作用增加斑块形成,促进氧化应激、内皮功能障碍和成骨分化[6]
BMP-2 在人钙化瓣膜的骨化区域过表达B 淋巴细胞和 T 淋巴细胞密集浸润区域的肌成纤维细胞和前成骨细胞[2]
它是动脉粥样硬化血管钙化与正常骨形成机制之间的纽带 [3]
BMP-2 诱导血管生成、内皮细胞 (EC) 增殖和迁移[7]
BMP-2 还能增强血管生成、内皮细胞 (EC) 增殖和迁移[7]。成骨细胞和软骨细胞主转录调节因子 RUNX2 的作用,以依赖于氧化应激和内质网 (ER) 应激的方式促进培养的人冠状血管 SMC 的矿化[8]

体外研究
(In Vitro)

BMP-2 (10 nM; 15 min) shows little effect on the phosphorylation of MADR1, indicating MADR1 is a downstream component in the BMP2 signal transduction pathway[9].
BMP-2 (50 ng/mL; 14 d) induces osteogenic differentiation of mesenchymal stem cells (MSCs) as well as strong synergistic effect with 1 ng/mL VEGF and 10 ng/mL bFGF, in bone marrow of femurs and tibias of 6 week-old rats[10].

生物活性

1. Measured by its ability to induce alkaline phosphatase production by ATDC-5 Cells. The ED50 for this effect is typically <0.2 µg/mL.
2. Measured by its ability to induce alkaline phosphatase production by C2C12 cells. The ED50 for this effect is typically <1 µg/mL.

种属

Human; Rat; Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

P12643 (Q283-R396)

基因 ID

650  [NCBI]

蛋白结构
N-term
BMP-2 (Q283-R396)
Accession # P12643
C-term
中文名
重组骨形态发生蛋白 2
同用名
rHuBMP-2; BMP2A; BMP-2A; BMP2
氨基酸序列

QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

分子量

Approximately 26 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against 50 mM acetic acid.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 20 mM HAc or diluted with 5 mM HCl.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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