BMP-2 蛋白, Human/Mouse/Rat (P. pastoris, His)

BMP-2 protein is an important member of the TGF-β superfamily and is critical in cardiogenesis, neurogenesis, and osteogenesis, inducing cartilage and bone formation. It initiates canonical BMP signaling by binding to BMPR1A and BMPR2, triggering BMPR2 phosphorylation and SMAD1/5/8 activation for gene transcription regulation. BMP-2 Protein, Human/Mouse/Rat (P. pastoris, His) is the recombinant human-derived BMP-2 protein, expressed by P. pastoris , with N-6*His labeled tag.

BMP-2 蛋白是 TGF-β 超家族的重要成员，在多种发育过程中发挥着重要作用，包括心脏生成、神经生成和骨生成。它诱导软骨和骨形成，并通过与 I 型受体 BMPR1A 和 II 型受体 BMPR2 结合启动典型的 BMP 信号级联。这种复合物的形成触发 BMPR2 磷酸化，激活 BMPR1A，进而磷酸化 SMAD1/5/8 以调节基因转录。BMP-2 还参与非经典途径，例如 ERK/MAP 激酶信号级联，影响成骨细胞分化。此外，它还通过 EIF2AK3-EIF2A-ATF4 途径刺激成肌细胞分化为成骨细胞。作为成牙本质细胞分化的正调节因子，BMP-2 形成同二聚体并与多种蛋白质相互作用，包括 SOSTDC1、GREM2、RGMA、RGMB、RGMC、ASPN、FBN1、FBN2、SCUBE3、TNFAIP6 和 ERFE。其复杂的相互作用凸显了其在多个细胞过程中的多功能调节作用。

Human

P. pastoris

N-6*His

P12643 (Q283-R396)

650  [NCBI]

QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

17 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.