1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules Macrophage CD Proteins
  4. BTLA BTLA/CD272
  5. BTLA/CD272 蛋白, Rat (HEK293, mFc)

BTLA/CD272 蛋白, Rat (HEK293, mFc)

目录号: HY-P75466
COA 产品使用指南 技术支持

BTLA/CD272 蛋白通过 PTPN6/SHP-1 和 PTPN11/SHP-2 抑制抗原受体信号传导。它通过与 TNFRSF14 的顺式和反式相互作用来调节免疫反应。在顺式中,它维持初始 T 细胞的静息状态,而在反式中,它支持效应 T 细胞中的生存信号。BTLA/CD272 与 PTPN6/SHP-1、PTPN11/SHP-2 和 TNFRSF14/HVEM 相互作用。BTLA/CD272 蛋白, Rat (HEK293, mFc) 是重组的 BTLA/CD272 蛋白,由 HEK293 表达,带有 C-mFc 标签。BTLA/CD272 蛋白, Rat (HEK293, mFc) 全长 154 个氨基酸,分子量约为 ~55-77 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

BTLA/CD272 protein inhibits antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. It regulates immune responses through cis and trans interactions with TNFRSF14. In cis, it maintains resting state in naive T cells, while in trans, it supports survival signals in effector T cells. BTLA/CD272 interacts with PTPN6/SHP-1, PTPN11/SHP-2, and TNFRSF14/HVEM. BTLA/CD272 Protein, Rat (HEK293, mFc) is the recombinant rat-derived BTLA/CD272 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of BTLA/CD272 Protein, Rat (HEK293, mFc) is 154 a.a., with molecular weight of ~55-77 kDa.

研究背景

BTLA/CD272 蛋白作为淋巴细胞的抑制性受体,通过 PTPN6/SHP-1 和 PTPN11/SHP-2 对抗原受体信号传导发挥负调节作用。它与 TNFRSF14 发生顺式和反式相互作用。在顺式相互作用中,BTLA/CD272发挥免疫调节作用,抑制初始T细胞中的反式相互作用以维持静息状态。相反,在反式相互作用中,它可以在适应性免疫反应中占主导地位,为效应 T 细胞提供生存信号。该蛋白与酪氨酸磷酸酶 PTPN6/SHP-1 和 PTPN11/SHP-2 相互作用,还通过其富含半胱氨酸的结构域 1 与 TNFRSF14/HVEM 结合。

生物活性

Measured by its binding ability in a functional ELISA. When Mouse HVEM/TNFRSF14 is immobilized at 0.5 μg/mL(100 μL/well)can bind Recombinant Rat BTLA. The ED50 for this effect is approximately 1.083 μg/mL.

  • Measured by its binding ability in a functional ELISA. When Mouse HVEM/TNFRSF14 is immobilized at 0.5 μg/mL(100 μL/well)can bind Recombinant Rat BTLA. The ED50 for this effect is approximately 1.083 μg/mL.
种属

Rat

表达系统

HEK293

标签

C-mFc

蛋白编号

Q6PNM1 (K30-Y183)

基因 ID
蛋白结构
N-term
BTLA (K30-Y183)
Accession # Q6PNM1
mFc
C-term
同用名
B- and T-lymphocyte attenuator; CD272; BTLA
氨基酸序列

KEPTKRIGEECRVQLKIKRNSSRSAWTGELFKIECPVTYCVHRPNVTWCKHNGTRCVPLEVGPQLHTSWVENDQASAFVLYFEPIHLSDDGVYTCSANLNSEVINSHSVVIHVTERTQNCSEHPLITASDIPDATNASRPSTMEERPGRTWLLY

分子量

Approximately 55-77 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BTLA/CD272 Protein, Rat (HEK293, mFc)
目录号:
HY-P75466
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