Calcitonin/CALCA 蛋白, Human (His)

Calcitonin Protein, Human (His), is a recombinant human Calcitonin expressed in E. coli with a His tag at the N-terminus. Calcitonin has an effect on decreasing osteoclast activity and has the potential for hypercalcemia research^[1].

降钙素是一种由 32 个氨基酸组成的激素，由甲状腺 C 细胞分泌。血清钙浓度增加会刺激降钙素分泌，降钙素可防止高钙血症的发生。降钙素也会受到胃泌素等胃肠激素的刺激^[1]。

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse chondrogenic cells. The ED₅₀ for this effect is 0.8463 µg/mL, corresponding to a specific activity is 1181.614 units/mg.

Human

E. coli

C-6*His

P01258-1 (Y58-N141)

796

YVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN

Approximately 15.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.2, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.