Calreticulin/CALR 蛋白, Human (His)

The calreticulin/CALR protein is a calcium-binding molecular chaperone that promotes ER folding and quality control. It interacts with monoglucosylated glycoproteins and promotes nuclear export of NR3C1. Calreticulin/CALR Protein, Human (His) is the recombinant human-derived Calreticulin/CALR protein, expressed by E. coli , with N-6*His labeled tag. The total length of Calreticulin/CALR Protein, Human (His) is 400 a.a., with molecular weight of ~50.6 kDa.

钙网蛋白/CALR 蛋白是一种钙结合伴侣，通过促进折叠、寡聚体组装和质量控制在内质网 (ER) 中发挥着至关重要的作用。它通过钙网蛋白/钙连接蛋白循环实现这一点，在该循环中，它与内质网中合成的几乎所有单葡萄糖基化糖蛋白瞬时相互作用。此外，钙网蛋白/CALR 蛋白与 NR3C1 的 DNA 结合域相互作用，促进其核输出。它还参与母体基因表达的调节，并可能通过调节钙稳态参与卵母细胞的成熟。在卵母细胞激活过程中，钙网蛋白/CALR 蛋白从皮质颗粒中胞吐出来，并可能有助于阻止多精受精。它以单体形式存在，是由各种蛋白质（例如 CELF1/CUGBP1、CALR、CALR3、EIF2S1、EIF2S2、HSP90B1 和 HSPA5）组成的 EIF2 复合体的一部分。钙网蛋白/CALR 蛋白与多种其他蛋白相互作用，包括 PDIA3/ERp57、SPACA9、TRIM21、NR3C1、PPIB、PDIA5、GABARAP、HLA-E-B2M、HLA-G-B2M 复合物、HLA-F 和 CLCC1。

Human

E. coli

N-6*His

P27797 (E18-L417)

811  [NCBI]

EPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKDPDASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQIDNPDYKGTWIHPEIDNPEYSPDPSIYAYDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL

Approximately 50.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.