Can f 4 蛋白, Canine (HEK293, His)

Can f 4 Protein, Canine (HEK293, His), an IgE-binding dog dander protein, is an important respiratory allergen. Can f 4 Protein is a dog allergen of the lipocalin family^[1]. Can f 4 Protein, Canine (HEK293, His) is the recombinant canine-derived Can f 4 protein, expressed by HEK293 , with C-His, C-6*His labeled tag.

狗毛屑是呼吸道过敏的常见原因，症状包括鼻炎、结膜炎和支气管哮喘。狗毛和皮屑提取物含有复杂的过敏性和非过敏性蛋白质。迄今为止，脂质运载蛋白家族的 4 种狗过敏原 Canf1、Canf2、Canf4 和 Canf6，以及狗血清白蛋白 Canf3 和狗前列腺激肽释放酶 Canf5 的 IgE 反应性已得到详细表征。配体结合袋的大小和氨基酸组成表明 Canf4 仅能够结合相对较小的疏水性分子，Canf4 的晶体结构包含过敏原的单体和二聚体形式，表明 Canf4 能够形成瞬时（弱）二聚体。当四个 β 链的末端与第二个单体的相同链堆积在一起时，就形成了 Canf4 的二聚体结构^[1][2]。

Canine

HEK293

C-His;C-6*His

NP_001177855.1 (Q17-E174)

100463491  [NCBI]

QLPLPNVLTQVSGPWKTLYISSNNLDKIGDNGPFRIYMRGINVDIPRLKMSFNFYVKVDGECVENSVGASIGRDNLIKGEYNGGNYFRIIDMTPNALIGYDVNVDSKGKITKVALLMGRGAHVNEEDIAKFKKLSREKGIPEENIIYLGDTDNCPNHE

Approximately 19 kDa.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.