1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. CANT1 蛋白, Human (HEK293, Fc)

CANT1 蛋白, Human (HEK293, Fc)

目录号: HY-P74356
产品使用指南

CANT1是一种钙依赖性核苷酸酶,对UDP表现出明显的偏好,其酶活性顺序为UDP > GDP > UTP > GTP。值得注意的是,它对ADP活性很小,对ATP活性更小,但不水解AMP和GMP。CANT1 蛋白, Human (HEK293, Fc) 是重组的 CANT1 蛋白,由 HEK293 表达,带有 N-hFc 标签。

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描述

CANT1 is a calcium-dependent nucleotidase that shows a clear preference for UDP, and the order of its enzymatic activity is UDP > GDP > UTP > GTP. It is worth noting that it has little activity on ADP and even less activity on ATP, but it does not hydrolyze AMP and GMP. CANT1 Protein, Human (HEK293, Fc) is the recombinant human-derived CANT1 protein, expressed by HEK293 , with N-hFc labeled tag.

研究背景

CANT1是一种钙依赖性核苷酸酶,对UDP作为底物表现出强烈的偏好,其活性顺序为UDP > GDP > UTP > GTP。值得注意的是,它对 ADP 的活性非常低,对 ATP 的活性甚至更低,但它不会水解 AMP 和 GMP。这种酶谱表明在核苷酸代谢中具有特定作用。CANT1 参与蛋白聚糖合成,表明它参与与这些重要大分子的修饰和调节相关的过程。CANT1 的钙依赖性性质凸显了其通过细胞内钙水平的调节,为其酶功能和潜在的细胞作用增加了一层额外的复杂性。

种属

Human

表达系统

HEK293

标签

N-hFc

蛋白编号

Q8WVQ1-1 (G80-I401)

基因 ID
蛋白结构
N-term
hFc
CANT1 (G80-I401)
Accession # Q8WVQ1-1
C-term
同用名
Soluble calcium-activated nucleotidase 1; SCAN-1; CANT1; SHAPY
分子量

Approximately 65 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CANT1 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CANT1 Protein, Human (HEK293, Fc)
目录号:
HY-P74356
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