CAPZA2 蛋白, Human (His-SUMO)

The CAPZA2 protein is a member of the F-actin capping protein family and binds to the barbed ends of actin filaments, preventing subunit exchange without severing the filaments. CAPZA2 Protein, Human (His-SUMO) is the recombinant human-derived CAPZA2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of CAPZA2 Protein, Human (His-SUMO) is 285 a.a., with molecular weight of ~48.8 kDa.

CAPZA2 蛋白是 F-肌动蛋白加帽蛋白的一员，以不依赖 Ca(2+) 的方式与肌动蛋白丝快速生长的末端（有倒刺的末端）结合，有效地阻断这些末端的亚基交换，而不会切断肌动蛋白丝，将其与其他加帽蛋白（如凝溶胶蛋白和塞维林）区分开来。CAPZA2 作为具有 α 和 β 亚基的异二聚体发挥作用，并且是 WASH 复合物的组成部分，该复合物包含 F-肌动蛋白帽蛋白亚基 α（CAPZA1、CAPZA2 或 CAPZA3）、F-肌动蛋白帽蛋白亚基 β（ CAPZB) 和各种 WASH 复合亚基。此外，CAPZA2 与 RCSD1/CAPZIP 相互作用，并直接与 CRACD 结合，这种相互作用显示出减少其与肌动蛋白的结合。CAPZA2 在调节肌动蛋白动力学方面的多功能性突出了其在调节细胞骨架结构和与肌动蛋白丝组织相关的细胞过程中的作用。

Human

E. coli

N-6*His;N-SUMO

P47755 (A2-A286)

830  [NCBI]

ADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA

Approximately 48.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.