1. 重组蛋白
  2. CAR-T related Proteins Enzymes & Regulators
  3. Lyases (EC 4) Carbonic Anhydrase
  4. Carbonic Anhydrase 9 (CA IX)
  5. Carbonic Anhydrase 9 蛋白, Human (HEK293, His)

Carbonic Anhydrase 9 蛋白, Human (HEK293, His)

目录号: HY-P70064
COA 产品使用指南

碳酸酐酶 9 (CA9) 蛋白作为催化剂在二氧化碳和水的转化中发挥着至关重要的作用。它促进碳酸氢根和氢离子(碳酸的离解离子)的形成。Carbonic Anhydrase 9 蛋白, Human (HEK293, His) 是重组的 碳酸酐酶 9 蛋白,由 HEK293 表达,带有 C-6*His 标签。Carbonic Anhydrase 9 蛋白, Human (HEK293, His) 全长 377 个氨基酸,分子量为 45-60 kDa。

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规格 价格 是否有货 数量
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5 μg ¥560 In-stock
10 μg ¥950 In-stock
50 μg ¥2650 In-stock
100 μg ¥4500 询价
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The carbonic anhydrase 9 (CA9) protein plays a crucial role as a catalyst in the conversion of carbon dioxide and water. It promotes the formation of bicarbonate and hydrogen ions (dissociated ions of carbonic acid). Carbonic Anhydrase 9 Protein, Human (HEK293, His) is the recombinant human-derived Carbonic Anhydrase 9 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Carbonic Anhydrase 9 Protein, Human (HEK293, His) is 377 a.a., with molecular weight of 45-60 kDa.

研究背景

碳酸酐酶 9 (CA9) 蛋白作为二氧化碳和水转化过程中的催化剂发挥着至关重要的作用。它促进碳酸氢根和氢离子(碳酸的离解离子)的形成。这种酶活性对于维持适当的 pH 平衡和调节各种生理过程(包括酸碱稳态、呼吸和离子转运)至关重要。CA9 蛋白在二氧化碳和水的动态平衡中发挥着关键作用,有助于这些分子在体内的有效运输和调节。

生物活性

Measured by its esterase activity.The specific activity is 19.46-46.008 pmol/min/μg.as measured with under the described conditions.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q16790 (Q38-D414)

基因 ID

768  [NCBI]

蛋白结构
N-term
CA9 (Q38-D414)
Accession # Q16790
6*His
C-term
同用名
rHuCarbonic Anhydrase 9/CA9, His; CA9; CA-IX; Carbonic Anhydrase IX; Carbonate dehydratase IX; G250; MN; P54/58N; RCC; RCC-associated protein G250
氨基酸序列

QRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNSCLAAGD

分子量

Approximately 45-60 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Carbonic Anhydrase 9 Protein, Human (HEK293, His)
目录号:
HY-P70064
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