Carboxypeptidase B2/CPB2 蛋白, Human (HEK293, His)

Carboxypeptidase B2 Protein, Human (HEK293, His) is an approximately 47.0 kDa human carboxypeptidase B2 with a His-flag. Carboxypeptidase B2 is a enzyme belonging to the peptidase M14 family and hydrolyze C-terminal peptide bonds^[1].

羧肽酶 B2 是肽酶 M14 家族的分泌型糖蛋白成员。它由肝细胞和血小板表达，其分子量差异归因于糖基化。CPB2 可以被凝血酶和纤溶酶裂解，产生相对不溶的非糖基化酶活性片段 (TAFIa)。活性 CPB2 可去除纤维蛋白 C 端赖氨酸残基，从而中断纤溶酶生成并促进纤维蛋白聚合。
人 CPB2 前体长度为 401 个氨基酸 (aa)。它包含一个原序列 (aa 23-114) 和一个作用于 C 端 Lys 或 Arg 残基的活性片段 (aa 115-423)^[1][2] 。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

Q96IY4 (F23-V423)

1361  [NCBI]

FQSGQVLAALPRTSRQVQVLQNLTTTYEIVLWQPVTADLIVKKKQVHFFVNASDVDNVKAHLNVSGIPCSVLLADVEDLIQQQISNDTVSPRASASYYEQYHSLNEIYSWIEFITERHPDMLTKIHIGSSFEKYPLYVLKVSGKEQAAKNAIWIDCGIHAREWISPAFCLWFIGHITQFYGIIGQYTNLLRLVDFYVMPVVNVDGYDYSWKKNRMWRKNRSFYANNHCIGTDLNRNFASKHWCEEGASSSSCSETYCGLYPESEPEVKAVASFLRRNINQIKAYISMHSYSQHIVFPYSYTRSKSKDHEELSLVASEAVRAIEKISKNTRYTHGHGSETLYLAPGGGDDWIYDLGIKYSFTIELRDTGTYGFLLPERYIKPTCREAFAAVSKIAWHVIRNVHHHHHH

Approximately 58.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM ZnCl₂, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. D Leenaerts, et al. Carboxypeptidase U (CPU, carboxypeptidase B2, activated thrombin-activatable fibrinolysis inhibitor) inhibition stimulates the fibrinolytic rate in different in vitro models. J Thromb Haemost. 2018 Oct;16(10):2057-2069.  [Content Brief]

  [2]. D Leenaerts, et al. Plasma levels of carboxypeptidase U (CPU, CPB2 or TAFIa) are elevated in patients with acute myocardial infarction. J Thromb Haemost. 2015 Dec;13(12):2227-32.  [Content Brief]