Cathepsin D 蛋白, Human (HEK293, C-His)

Cathepsin D protein is an acidic protease that actively participates in intracellular protein breakdown. Studies have shown that it is critical in the processing of amyloid precursor protein (APP), with ADAM30-mediated cleavage and activation leading to APP degradation. Cathepsin D Protein, Human (HEK293, C-His) is the recombinant human-derived Cathepsin D protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cathepsin D Protein, Human (HEK293, C-His) is 392 a.a., with molecular weight of ~44.78 kDa.

组织蛋白酶 D 是一种酸性蛋白酶，在细胞内蛋白质分解中发挥其活性。研究证明，这种蛋白酶参与淀粉样前体蛋白 (APP) 的加工，ADAM30 对其进行切割和激活，导致 APP 降解。组织蛋白酶 D 的参与延伸到包括乳腺癌在内的各种疾病的发病机制，并且被认为在阿尔茨海默病中发挥作用，突出了其在细胞过程中的重要性以及与病理状况的潜在联系。

Measured by its ability to cleave a peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is 4624.96 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P07339 (L21-L412)

1509  [NCBI]

LVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARLHHHHHH

Approximately 44.78 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.