Cathepsin D 蛋白, Mouse (HEK293, His)

Cathepsin D Protein, Mouse (HEK293, His) is an approximately 56.0 kDa mouse cathepsin D with a His tag. Cathepsin D is a lysosomal aspartic protease of the pepsin family^[1].

组织蛋白酶 D 是一种溶酶体天冬氨酸蛋白酶，属于胃蛋白酶家族。小鼠组织蛋白酶 D 由信号肽（残基 120）、前肽（残基 2164）和成熟链（残基 65410）（13）组成。组织蛋白酶 D 在大多数细胞中表达，并在某些癌症（乳腺癌、黑色素瘤、卵巢癌等）中过度表达。组织蛋白酶 D 会增加临床转移的发生率，涉及细胞生长增加和接触抑制减少，而不是癌细胞通过基底膜逃逸^[1][2]。
组织蛋白酶 D 在酸性 pH 下激活后充当蛋白酶，或在中性 pH 下充当不同膜受体的配体^[1]。

Measured by its ability to cleave a peptide substrate, Mca-PLGL-Dpa-AR-NH2. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read). The specific activity is 5026.88 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

C-6*His

P18242 (I21-L410)

13033  [NCBI]

IIRIPLRKFTSIRRTMTEVGGSVEDLILKGPITKYSMQSSPKTTEPVSELLKNYLDAQYYGDIGIGTPPQCFTVVFDTGSSNLWVPSIHCKILDIACWVHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCKSDQSKARGIKVEKQIFGEATKQPGIVFVAAKFDGILGMGYPHISVNNVLPVFDNLMQQKLVDKNIFSFYLNRDPEGQPGGELMLGGTDSKYYHGELSYLNVTRKAYWQVHMDQLEVGNELTLCKGGCEAIVDTGTSLLVGPVEEVKELQKAIGAVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPDKYILKVSQGGKTICLSGFMGMDIPPPSGPLWILGDVFIGSYYTVFDRDNNRVGFANAVVLHHHHHH

Approximately 46 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.  

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, pH 5.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. H Rochefort, et al.Cathepsin D in breast cancer: mechanisms and clinical applications, a 1999 overview. Clin Chim Acta. 2000 Feb 15;291(2):157-70.  [Content Brief]

  [2]. T Tsukuba, et al. New functional aspects of cathepsin D and cathepsin E. Mol Cells  [Content Brief]