1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Cathepsin
  4. Cathepsin X/Cathepsin Z
  5. Cathepsin Z/CTSZ 蛋白, Human (HEK293, C-His)

Cathepsin Z/CTSZ 蛋白, Human (HEK293, C-His)

目录号: HY-P7758A
COA 产品使用指南

研究证明,组织蛋白酶 Z/CTSZ 蛋白具有双重羧基单肽酶和羧基二肽酶活性。此外,它还通过产生激肽增强肽而显示出多功能性。Cathepsin Z/CTSZ 蛋白, Human (HEK293, C-His) 是重组的 组织蛋白酶 Z/CTSZ 蛋白,由 HEK293 表达,带有 C-6*His 标签。Cathepsin Z/CTSZ 蛋白, Human (HEK293, C-His) 全长 280 个氨基酸,分子量为 37-40 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Cathepsin Z/CTSZ Protein exhibits dual carboxy-monopeptidase and carboxy-dipeptidase activities, as evidenced by research. Additionally, it displays functional versatility by generating kinin potentiating peptides. Cathepsin Z/CTSZ Protein, Human (HEK293, C-His) is the recombinant human-derived Cathepsin Z/CTSZ protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cathepsin Z/CTSZ Protein, Human (HEK293, C-His) is 280 a.a., with molecular weight of 37-40 kDa.

研究背景

研究表明,组织蛋白酶 Z/CTSZ 蛋白具有羧基单肽酶和羧基二肽酶活性。它还具有产生激肽增强肽的能力,展示了其多功能性。

生物活性

Measured by its ability to cleave the fluorogenic peptide substrate, MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(DNP)-OH. The specific activity is 3210.6 pmol/min/µg, as measured under the described conditions.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9UBR2 (G24-V303)

基因 ID

1522

蛋白结构
N-term
CTSZ (G24-V303)
Accession # Q9UBR2
6*His
C-term
同用名
rHuCathepsin X, His; Cathepsin Z; Cathepsin P; Cathepsin X; CTSZ
氨基酸序列

GLYFRRGQTCYRPLRGDGLAPLGRSTYPRPHEYLSPADLPKSWDWRNVDGVNYASITRNQHIPQYCGSCWAHASTSAMADRINIKRKGAWPSTLLSVQNVIDCGNAGSCEGGNDLSVWDYAHQHGIPDETCNNYQAKDQECDKFNQCGTCNEFKECHAIRNYTLWRVGDYGSLSGREKMMAEIYANGPISCGIMATERLANYTGGIYAEYQDTTYINHVVSVAGWGISDGTEYWIVRNSWGEPWGERGWLRIVTSTYKDGKGARYNLAIEEHCTFGDPIVHHHHHH

分子量

37-40 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Cathepsin Z/CTSZ Protein, Human (HEK293, C-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cathepsin Z/CTSZ Protein, Human (HEK293, C-His)
目录号:
HY-P7758A
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