CBFB 蛋白, Human (His)

CBFB forms a heterodimeric complex core binding factor (CBF) with RUNX family proteins, which enhances the DNA binding ability of RUNX members (such as RUNX1, RUNX2, and RUNX3). Heterodimers bind to specific sites in enhancers and promoters to regulate the transcription of target genes. CBFB Protein, Human (His) is the recombinant human-derived CBFB protein, expressed by E. coli , with N-6*His labeled tag. The total length of CBFB Protein, Human (His) is 181 a.a., with molecular weight of ~25 kDa.

CBFB 是一种核心结合因子，与 RUNX 家族蛋白（包括 RUNX1、RUNX2 和 RUNX3）形成异二聚体复合物 CBF。RUNX 成员通过其 runt 结构域识别调控区内的核心共有结合序列 5'-TGTGGT-3'，或特殊情况下的 5'-TGCGGT-3'，从而对其靶基因进行转录调节。CBFB 作为非 DNA 结合调节亚基，可增强 RUNX 变构的序列特异性 DNA 结合能力。这些异二聚体结合各种增强子和启动子的核心位点，例如鼠白血病病毒、多瘤病毒增强子、T细胞受体增强子、LCK、IL3和GM-CSF启动子。CBF 复合物在细胞毒性 (CD8+) T 细胞发育过程中抑制 ZBTB7B 转录因子中发挥作用，通过与 ZBTB7B 基因座内的 RUNX 结合序列结合，充当转录沉默子，从而促进细胞毒性 T 细胞分化。这种相互作用涉及与 RUNX1、RUNX2 和 RUNX3 的异二聚化，并且 CBFB 还与 COPRS 相互作用并与 PRMT5 和 RUNX1 形成复合物。

Human

E. coli

N-6*His

Q13951-1 (P2-P182)

865  [NCBI]

PRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLDGMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQLLAVTGKKTTRP

Approximately 25 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of sterile 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.