1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. CBS 蛋白, Human (His)

CBS 蛋白, Human (His)

目录号: HY-P72118
COA 产品使用指南

CBS 是一种氢裂解酶,通过催化 β-置换反应启动转硫途径,利用 L-同型半胱氨酸将 L-丝氨酸转化为 L-胱硫醚。该过程消除了 L-蛋氨酸和潜在有害的代谢物 L-同型半胱氨酸。CBS 除了其分解代谢作用外,还有助于硫化氢的产生,作为具有信号传导和细胞保护作用的气体递质,特别是对神经元。CBS 蛋白, Human (His) 是重组的 CBS 蛋白,由 E. coli 表达,带有 N-6*His 标签。CBS 蛋白, Human (His) 全长 550 个氨基酸,分子量约为 ~64.5 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CBS, a hydro-lyase, initiates the transsulfuration pathway by catalyzing the beta-replacement reaction, converting L-serine to L-cystathionine using L-homocysteine. This process eliminates L-methionine and the potentially harmful metabolite L-homocysteine. CBS, beyond its catabolic role, contributes to hydrogen sulfide production, serving as a gasotransmitter with signaling and cytoprotective effects, particularly on neurons. CBS Protein, Human (His) is the recombinant human-derived CBS protein, expressed by E. coli , with N-6*His labeled tag. The total length of CBS Protein, Human (His) is 550 a.a., with molecular weight of ~64.5 kDa.

研究背景

CBS 是一种氢裂解酶,通过催化 β-取代反应(其中 L-丝氨酸的羟基被 L-同型半胱氨酸取代),在启动转硫途径中发挥关键作用,从而形成 L-胱硫醚(L-胱硫醚的前体)。L-半胱氨酸。这种酶促过程有助于消除 L-蛋氨酸和潜在有害的代谢物 L-同型半胱氨酸。除了在分解代谢中的作用外,CBS 也是硫化氢产生的重要组成部分,作为一种气体递质,具有信号传导和细胞保护作用,特别是对神经元。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P35520 (P2-K551)

基因 ID

875  [NCBI]

蛋白结构
N-term
6*His
CBS (P2-K551)
Accession # P35520
C-term
同用名
AI047524; AI303044; Beta thionase; Beta-thionase; Cbs; Cbs cystathionine beta-synthase; CBS_HUMAN; Cystathionine beta synthase; Cystathionine beta-synthase; EC 4.2.1.22; HIP 4; HIP4; Methylcysteine synthase; MGC18856; MGC18895; MGC37300; OTTHUMP00000109416; OTTHUMP00000109418; Serine sulfhydrase
氨基酸序列

PSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWIRPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIEPTSGNTGIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK

分子量

Approximately 64.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

CBS Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CBS Protein, Human (His)
目录号:
HY-P72118
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