RANTES/CCL5 蛋白, Human

RANTES/CCL5 Protein is a secreted protein located outside the cell membrane and is a key pro-inflammatory chemokine and immune regulatory molecule in the CC chemokine family. RANTES/CCL5 Protein sends signals through its specific G protein-coupled receptors (GPCR) CCR1, CCR3, and CCR5, mediating inflammatory immune responses, viral infections, and tumor development. RANTES/CCL5 Protein can promote endothelial cell migration, proliferation, and angiogenesis in rats. RANTES/CCL5 Protein is useful for research into tumors, autoimmune diseases, and atherosclerosis. RANTES/CCL5 Protein, Human is a recombinant human CCL5 (S24-S91) protein expressed by E. coli^[1][2].

CCL5，也称为 RANTES（正常 T 细胞激活、表达和分泌的调节），属于趋化因子 CC 亚家族。CCL5 基因位于人类 17 号染色体 q11.2-q12 区域，编码分子量为 8 kDa 的 CCL5 蛋白^[1]。
RANTES/CCL5 Protein 可以在许多细胞类型分泌，如血小板、内皮细胞、平滑肌细胞、巨噬细胞和活化的 T 细胞^[1]。
RANTES/CCL5 Protein 可以通过利用硫酸肝素蛋白聚糖 (HSPGs) 和/或在 PKCδ-JNK/ERK 通路中选择性激活 PKCδ、JNK 和 ERK 蛋白，从而诱导人类风湿关节炎滑膜成纤维细胞中的 MMP-1 和 MMP-13 表达，最终诱导胶原降解^[3]。
RANTES/CCL5 Protein 可以通过与 GPCR、CCR1 和 CCR5 结合，与其蛋白聚糖受体 SDC-1、SDC-4 和 CD-44 结合，并部分依赖内皮细胞分泌的 VEGF，从而具有促血管生成作用^[1]。
RANTES/CCL5 Protein 由 RSV 感染小鼠中的相关细胞因子 IL-13 诱导产生，从而导致气道高反应性 (AHR)，加重气道疾病^[4]。
RANTES/CCL5 Protein 通过和 CCR5 相互作用，并在 MEK/ERK 通路中增加 MEK 和 ERK 的磷酸化和激活 NF-κB，导致 αvβ3 整合素的激活并促进人骨肉瘤细胞的迁移^[5]。
RANTES/CCL5 Protein 的寡聚化形式在部分功能发挥中很重要，RANTES/CCL5 Protein 可以在中性 pH 环境中形成较大的聚集体，在降低 pH 值时形成较小的聚集体^[6]。
RANTES/CCL5 Protein 与多种病理过程有关，包括病毒感染、动脉粥样硬化、炎症性疾病、自身免疫性疾病和肿瘤^[1]。

RANTES/CCL5 Protein (1 或 10 nM, 25 天) 在大鼠椎间盘血管生成模型中诱导血管形成^[1]。
RANTES/CCL5 Protein (100 ng/mL, 24 h) 可以激活人类风湿关节炎滑膜成纤维细胞中 MMP-1 和 MMP-13 的表达从而诱导胶原降解^[3]。
RANTES/CCL5 Protein (3 ng/mL, 24 h) 可以促进人骨肉瘤细胞中 αvβ3 整合素的表达和细胞迁移^[6]。
RANTES/CCL5 Protein 表达缺陷的 Rantes KO 干细胞/祖细胞中 mTOR 活性降低，从而导致更年轻的造血干细胞表型^[7]。

RANTES/CCL5 Protein 在原发性呼吸道合胞病毒感染小鼠中被诱导产生，从而加重气道疾病^[4]。
RANTES/CCL5 Protein 在 Rantes 基因敲除 (KO) 小鼠中的表达缺乏可以导致髓系偏向的 HSC 和髓系祖细胞减少，T 细胞和淋巴样偏向的 HSC 增加^[7]。

1.Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10.0 ng/mL.
2.Measured by its ability to chemoattract THP-1 cells. The ED₅₀ for this effect is 2.640-4.751 ng/mL.

• 
  Measured by its ability to chemoattract THP-1 cells. The ED₅₀ for this effect is 4.751 ng/mL, corresponding to a specific activity is 2.105×10⁵ U/mg.

Human

E. coli

Tag Free

P13501 (S24-S91)

6352  [NCBI]

SPYSSDTTPCCFAYIARPLPRAHIKEYFYTSGKCSNPAVVFVTRKNRQVCANPEKKWVREYINSLEMS

7-12 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 6% Trehalose, 4% Mannitol, 0.05% Tween 80, pH 4.0 or 4mM HCL, pH 2.5 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Suffee N, et al. RANTES/CCL5-induced pro-angiogenic effects depend on CCR1, CCR5 and glycosaminoglycans. Angiogenesis. 2012 Dec;15(4):727-44.  [Content Brief]

  [2]. Maillard L, et al. RANTES/CCL5 mediated-biological effects depend on the syndecan-4/PKCα signaling pathway. Biol Open. 2014 Sep 26;3(10):995-1004.  [Content Brief]

  [3]. Agere SA, et al. RANTES/CCL5 Induces Collagen Degradation by Activating MMP-1 and MMP-13 Expression in Human Rheumatoid Arthritis Synovial Fibroblasts. Front Immunol. 2017 Oct 18;8:1341.  [Content Brief]

  [4]. Tekkanat KK, et al. RANTES (CCL5) production during primary respiratory syncytial virus infection exacerbates airway disease. Eur J Immunol. 2002 Nov;32(11):3276-84.  [Content Brief]

  [5]. Wang SW, et al. CCL5 and CCR5 interaction promotes cell motility in human osteosarcoma. PLoS One. 2012;7(4):e35101.  [Content Brief]

  [6]. Wang X, et al. Oligomeric structure of the chemokine CCL5/RANTES from NMR, MS, and SAXS data. Structure. 2011 Aug 10;19(8):1138-48.  [Content Brief]

  [7]. Ergen AV, et al. Rantes/Ccl5 influences hematopoietic stem cell subtypes and causes myeloid skewing. Blood. 2012 Mar 15;119(11):2500-9.  [Content Brief]