CCL6 蛋白, Mouse

CCL6 Protein, Mouse is a CC chemokine found only in rodents and is associated with macrophage infiltration in chronic inflammation as well as a role in tumorigenesis. CCL6 Protein, Mouse is a recombinant mouse CCL6 (G22-A116) expressed by E. coli^[1].

CCL6属于趋化因子CC家族，位于小鼠11号染色体上。CCL6可以在小鼠中性粒细胞和巨噬细胞系的细胞中表达，并且可以在适合骨髓细胞分化的条件下被大量诱导。C10/CCL6 与其他趋化因子具有显着的同源性，包括人 MIP-1γ、CC 趋化因子 F-18 (CCF-18)、血滤液 CC 趋化因子 (HCC-1) 和 HCC-2^[1] 。CCL6存在于多种慢性炎症性疾病中，包括实验性脱髓鞘疾病、过敏性气道炎症、博来霉素诱导的肺纤维化和慢性腹膜炎。CCL6 还可用作小鼠急性腹膜炎的单核细胞趋化剂^[2]。

CCL6 (100 -200 ng/mL) can cause concentration-dependent migration of murine ID8 cell lines and can induce migration through activation of ERK and PI3-kinase pathways, thereby enhancing downstream signaling through MYO9A and p-Cofilin^[3].

Full biological activity determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-405 ng/ml.

• 
  Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 404.9 ng/mL, corresponding to a specific activity is 2.470×10³ U/mg.

Mouse

E. coli

Tag Free

P27784 (G22-A116)

20305  [NCBI]

GLIQEIEKEDRRYNPPIIHQGFQDTSSDCCFSYATQIPCKRFIYYFPTSGGCIKPGIIFISRRGTQVCADPSDRRVQRCLSTLKQGPRSGNKVIA

Approximately 10.7 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM Tris, pH 8.0, 500 mM NaCl or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Bing Ma, et al. The C10/CCL6 chemokine and CCR1 play critical roles in the pathogenesis of IL-13-induced inflammation and remodeling. J Immunol. 2004 Feb 1;172(3):1872-81.  [Content Brief]

  [2]. Andrew M LaFleur, et al. Role of CC chemokine CCL6/C10 as a monocyte chemoattractant in a murine acute peritonitis. Mediators Inflamm. 2004 Dec;13(5-6):349-55.  [Content Brief]

  [3]. Venkatesh Krishnan, et al. Omental macrophages secrete chemokine ligands that promote ovarian cancer colonization of the omentum via CCR1. Commun Biol. 2020 Sep 22;3(1):524.  [Content Brief]