CCN1/Cyr61 蛋白, Human (HEK293, Fc)

CCN1/Cyr61 proteins play critical roles in cellular processes, promoting cell proliferation, chemotaxis, angiogenesis, and adhesion. It coordinates wound healing by upregulating key genes related to angiogenesis, inflammation and matrix remodeling in skin fibroblasts and is heparin binding dependent. CCN1/Cyr61 Protein, Human (HEK293, Fc) is the recombinant human-derived CCN1/Cyr61 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CCN1/Cyr61 Protein, Human (HEK293, Fc) is 357 a.a., with molecular weight of 71-80 kDa.

CCN1/Cyr61 蛋白在细胞过程中发挥多方面的作用，积极促进细胞增殖、趋化性、血管生成和粘附。它通过协调与皮肤成纤维细胞中血管生成、炎症和基质重塑相关的关键基因的上调，证明了对伤口愈合的关键参与。CCN1 的基因调节作用依赖于肝素结合。此外，它还通过下调 1 型胶原蛋白亚基 alpha-1 和 alpha-2 的表达来发挥调节控制作用。该蛋白通过整合素 α-6/β-1 促进细胞粘附和信号传导，通过整合素 α-v/β-5 促进细胞迁移，并通过整合素 α-v/β-3 刺激细胞增殖。它与整合素的相互作用是上下文相关的，依赖于肝素和特定的细胞类型。在皮肤成纤维细胞中，CCN1 结合 ITGA6/ITGB1，在内皮细胞中，它结合 ITGAV/ITGB3，在血小板中，它与 ITGA2B/ITGB3 结合，从而影响多种细胞功能。

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Recombinant Human CCN1 immobilized at 0.1 μg/mL, 100 μL/well, will mediate 19.19% BALB/3T3 cell adhesion (3 x 10⁴ cell/well added).

Human

HEK293

C-hFc

O00622 (T25-D381)

3491  [NCBI]

TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCDEDSIKDPMEDQDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTTSWSQCSKTCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCLSVKKYRPKYCGSCVDGRCCTPQLTRTVKMRFRCEDGETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFNDIHKFRD

68-80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM Tris-HCl, 6% Trehalose, 4% Mannitol, 0.05% Tween80, pH7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.