CD137/4-1BB 蛋白, Canine (HEK293, His)

4-1BB (CD137; TNFRSF9), is a surface glycoprotein, a receptor of TNFSF9/4-1BBL, belongs to the tumor necrosis factor (TNF) receptor superfamily^[1]. 4-1BB is helpful for T cell activation and development, and also induces peripheral mononuclear cell proliferation and migration to the tumor microenvironment^[2]. 4-1BB is also involved in enhancing Nrf2 and NF-κB pathway mediated apoptosis of endothelial cells^[3]. CD137/4-1BB Protein, Canine (HEK293, His) has 185 amino acids (M1-S185), produced by HEK293 cells with C-terminal His-tag.

4-1BB，由TNFRSF9（CD137，ILA）编码，属于肿瘤坏死因子（TNF）受体超家族。4-1BB是一种表面糖蛋白，在多种细胞中表达，例如T细胞、B细胞、自然杀伤（NK）细胞、树突状（DC）、嗜酸性粒细胞和肥大细胞；甚至多种肿瘤细胞如人类白血病细胞。广泛分布于血管平滑肌、肿瘤血管壁和肝细胞癌的肝组织中。4-1BB 优先选择 CD8+ 细胞，而不是 CD4+ 细胞。它提供共刺激信号并激活 CD8+ T 细胞的细胞毒性作用，并有助于形成记忆 T 细胞。最后，它促进免疫系统对抗肿瘤。此外，CD137 将 CD137L 与信号单核细胞结合，增加其存活、增殖并刺激其迁移和外渗。此外，它还诱导多种促炎因子的释放，导致炎症单核细胞涌入组织，形成炎症环境^[1]。具体来说，CD137通过上调Fra1的表达来促进单核细胞/巨噬细胞向肿瘤微环境的迁移。同时还促进单核细胞/巨噬细胞分化为破骨细胞，从而为乳腺癌细胞在骨中定植和生长提供有利的微环境。它为乳腺癌转移提供了一种有前景的治疗策略^[2]。此外，CD137 信号传导通过分别由 Nrf2 和 NF-κB 通路介导的促氧化和促炎症机制促进内皮细胞 (EC) 凋亡^[3]。4-1BB蛋白在人和小鼠体内的同源性较低，序列相似度为56.75%。

Immobilized Canine CD137 at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant Rat TNFSF9. The ED₅₀ for this effect is 1.722 µg/mL.

• 
  Immobilized Canine CD137 at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant Rat TNFSF9. The ED₅₀ for this effect is 1.722 µg/mL.

Canine

HEK293

C-His

XP_850336.1 (I24-S185)

608274  [NCBI]

IQDSCSKCPAGTFCGKNKSQICIPCPPNSFSSTSGQKACDICRQCEGVFRTKKVCSPISNAECECISGFHCLGAGCTMCEQDCKQGQELTKQGCKDCRFGTFNDQKHGICQPWTNCSLDGKSVLVNGTKESDAVCGPASAGFSPGTASATTPAPARDPGHTS

Approximately 24-34 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ye L, et al. CD137, an attractive candidate for the immunotherapy of lung cancer. Cancer Sci. 2020 May;111(5):1461-1467.  [Content Brief]

  [2]. Jiang P, et al. CD137 promotes bone metastasis of breast cancer by enhancing the migration and osteoclast differentiation of monocytes/macrophages. Theranostics. 2019 May 9;9(10):2950-2966.  [Content Brief]

  [3]. Geng T, et al. CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF-κB Pathway. Mediators Inflamm. 2020 Jun 6;2020:4321912.  [Content Brief]