1. 重组蛋白
  2. CAR-T related Proteins CD Antigens Fluorescent-labeled Recombinant Proteins
  3. CD19 B Cell CD Proteins Stem Cell CD Proteins Dendritic Cell CD Proteins
  4. CD19 蛋白, Human (FITC, HEK293, His)

CD19 蛋白, Human (FITC, HEK293, His)

目录号: HY-P701243
产品使用指南

CD19 蛋白是一种 B 细胞抗原受体辅助受体,可增强下游信号通路、降低激活阈值并帮助 B 细胞对抗原做出反应。它激活磷脂酰肌醇 3-激酶并动员细胞内 Ca(2+)。 CD19 对于 B-1 细胞分化至关重要,并影响 B 细胞增殖、免疫球蛋白水平和高亲和力抗体的产生。它与 CD21、CD81 和 CD225 复合,与 CD81 相互作用进行受体运输,并在磷酸化时与 VAV、GRB2、SOS、PLCG2、LYN 和磷脂酰肌醇 3-激酶的 p85 亚基结合。CD19 蛋白, Human (FITC, HEK293, His) 是重组的 CD19 蛋白,由 HEK293 表达,带有 C-His 标签。CD19 蛋白, Human (FITC, HEK293, His) 全长 272 个氨基酸,分子量为 68-72 kDa。

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描述

CD19 Protein, a B-cell antigen receptor coreceptor, enhances downstream signaling pathways, lowers activation threshold, and aids B-cell responses to antigens. It activates phosphatidylinositol 3-kinase and mobilizes intracellular Ca(2+). CD19 is essential for B-1 cell differentiation and influences B cell proliferation, immunoglobulin levels, and high-affinity antibody production. It complexes with CD21, CD81, and CD225, interacts with CD81 for receptor trafficking, and binds to VAV, GRB2, SOS, PLCG2, LYN, and p85 subunit of phosphatidylinositol 3-kinase when phosphorylated. CD19 Protein, Human (FITC, HEK293, His) is the recombinant human-derived CD19 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD19 Protein, Human (FITC, HEK293, His) is 272 a.a., with molecular weight of 68-72 kDa.

研究背景

CD19 蛋白作为 B 淋巴细胞上 B 细胞抗原受体复合物 (BCR) 的共受体,在降低下游信号通路激活阈值和促进 B 细胞对抗原的反应方面发挥着关键作用。它激活信号通路,导致磷脂酰肌醇 3-激酶的激活和细胞内 Ca(2+) 储备的动员。尽管 CD19 不是骨髓中 B 细胞分化早期步骤所必需的,但它对于 B-1 细胞的正常分化至关重要。此外,它对于正常 B 细胞响应抗原挑战的分化和增殖至关重要,影响血清免疫球蛋白水平和响应抗原挑战的高亲和力抗体的产生。 CD19 与成熟 B 细胞膜上的 CR2/CD21、CD81 和 IFITM1/CD225 形成复合物。它直接与 CD81 相互作用,这是活化 B 细胞表面 CD19 受体运输和区室化的关键相互作用。此外,当在特定酪氨酸残基上磷酸化时,CD19 与 VAV、GRB2、SOS、PLCG2、LYN 和磷脂酰肌醇 3-激酶的调节 p85 亚基相互作用。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P15391-1 (P20-K291)

基因 ID

930

蛋白结构
N-term
CD19 (P20-K291)
Accession # P15391-1
His
C-term
同用名
B-lymphocyte antigen CD19; CD19; B-lymphocyte surface antigen B4
分子量

68-72 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD19 Protein, Human (FITC, HEK293, His)
目录号:
HY-P701243
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