CD26/Dipeptidyl Peptidase 4 蛋白, Cynomolgus (HEK293, His)

CD26/Dipeptidyl Peptidase 4 Protein is a serine membrane anchored exopeptidase, belonging to dipeptidyl peptidase (DPP) family. CD26/Dipeptidyl Peptidase 4 Protein is widely expressed in a variety of tissues and many cells^[1]. CD26/Dipeptidyl Peptidase 4 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD26/Dipeptidyl Peptidase 4 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD26/Dipeptidyl Peptidase 4 Protein, Cynomolgus (HEK293, His) is 733 a.a., with molecular weight of 100-130 KDa.

二肽基肽酶 4 (DPP4)，也称为 T 细胞表面抗原 CD26，是人体中普遍存在的酶。广泛表达于多种组织（肺、脑、胰腺、肾、血管、前列腺、子宫、胸腺、淋巴结和脾）和多种细胞（上皮细胞、内皮细胞、免疫细胞），尤其是高表达 在肾脏和小肠。它是一种跨膜糖蛋白，由766个氨基酸组成，相对分子量为110kDa。其基本结构主要包括：胞内N端区、跨膜区和胞外区。胞外部分由柔性杆、糖基化丰富区（抗CD26抗体、腺苷脱氨酶（ADA）和caveolin-1的结合区）、半胱氨酸丰富区（胶原蛋白和纤连蛋白的结合区）和催化区组成 催化三联体 Ser630、Asp708 和 His740。其中，共翻译核心 N-糖基化与 DPP4 折叠和稳定性显着相关，而 N-末端唾液酸化似乎调节更多的病理生理过程。高唾液酸化的 DDP4 与 HIV 和类风湿性关节炎的发生有关，而唾液酸化不足的 DPP4 则与肺癌有关^[1]。

Measured by its ability to cleave 10 μM fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 3150.25 pmol/min/µg.

Cynomolgus

HEK293

C-10*His

F6VRB0 (D34-P766)

654491  [NCBI]

DATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSHRITSTGKEDMIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVQVPYPKAGAVNPTVKFFVVNTDSLSSATNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIETSTTGWVGRFRPSEPHFTSDGNSFYKIISNEEGYRHICYFQINKKNCTFITKGAWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGPRVLEDNSALDKMLQNVQMPSKTLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADAVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDAGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP

Approximately 100-130 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.