CD26/Dipeptidyl Peptidase 4 蛋白, Mouse (HEK293, Fc)

CD26/dipeptidyl peptidase 4 is a key cell surface receptor for T cell activation. It binds to ADA, CAV1, IGF2R and PTPRC to promote T cell proliferation and NF-kappa-B activation. CD26/Dipeptidyl Peptidase 4 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD26/Dipeptidyl Peptidase 4 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CD26/Dipeptidyl Peptidase 4 Protein, Mouse (HEK293, Fc) is 732 a.a., with molecular weight of ~122.5 kDa.

CD26/二肽基肽酶 4 蛋白作为细胞表面糖蛋白受体，对于 T 细胞受体 (TCR) 介导的 T 细胞激活中必需的共刺激信号至关重要。CD26 作为 T 细胞共激活的正调节因子，与 ADA、CAV1、IGF2R 和 PTPRC 结合。它与 CAV1 和 CARD11 的相互作用以 T 细胞受体/CD3 依赖性方式诱导 T 细胞增殖和 NF-kappa-B 激活。此外，CD26 与 ADA 的结合可调节淋巴细胞-上皮细胞粘附，而 CD26 与 FAP 的结合有助于细胞外基质 (ECM) 的细胞周蛋白水解，促进内皮细胞迁移和侵入 ECM。CD26 还可能在促进淋巴管内皮细胞粘附、迁移和管形成中发挥作用。当过度表达时，CD26 会增强细胞增殖，而这一过程会被 GPC3 抑制。除了其共激活作用之外，CD26 还充当具有二肽基肽酶活性的丝氨酸外肽酶，通过切割循环中的肽来调节各种生理过程，包括趋化因子、促有丝分裂生长因子、神经肽和肽激素，特别针对具有未取代 N 末端的肽。倒数第二个残基是脯氨酸。

Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). Read at excitation and emission wavelengths of 380 nm and 460 nm .The specific activity is 1362.0674 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

N-hFc

P28843 (S29-H760)

13482  [NCBI]

SKDEAAADSRRTYSLADYLKSTFRVKSYSLWWVSDFEYLYKQENNILLLNAEHGNSSIFLENSTFESFGYHSVSPDRLFVLLEYNYVKQWRHSYTASYNIYDVNKRQLITEEKIPNNTQWITWSPEGHKLAYVWKNDIYVKVEPHLPSHRITSTGEENVIYNGITDWVYEEEVFGAYSALWWSPNNTFLAYAQFNDTGVPLIEYSFYSDESLQYPKTVWIPYPKAGAVNPTVKFFIVNIDSLSSSSSAAPIQIPAPASVARGDHYLCDVVWATEERISLQWLRRIQNYSVMAICDYDKINLTWNCPSEQQHVEMSTTGWVGRFRPAEPHFTSDGSSFYKIISDKDGYKHICHFPKDKKDCTFITKGAWEVISIEALTSDYLYYISNQYKEMPGGRNLYKIQLTDHTNVKCLSCDLNPERCQYYAVSFSKEAKYYQLGCWGPGLPLYTLHRSTDHKELRVLEDNSALDRMLQDVQMPSKKLDFIVLNETRFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADASFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTLEVEDQIEAARQFVKMGFVDSKRVAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPIPEDNLDHYRNSTVMSRAEHFKQVEYLLIHGTADDNVHFQQSAQISKALVDAGVDFQAMWYTDEDHGIASSTAHQHIYSHMSHFLQQCFSLH

Approximately 122.5 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.