CD276/B7-H3 蛋白, Cynomolgus (HEK293, Fc)

CD276/B7-H3 protein can regulate T cell-mediated immune responses and act as a protective factor for tumor cells by inhibiting natural killer-mediated cell lysis. It also functions as a neuroblastoma cell marker, plays a role in acute and chronic transplant rejection, and modulates lymphocyte activity at mucosal surfaces. CD276/B7-H3 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived CD276/B7-H3 protein, expressed by HEK293 , with C-hFc labeled tag.

CD276/B7-H3 蛋白被认为在调节 T 细胞介导的免疫反应中发挥多方面的作用，通过抑制自然杀伤介导的细胞裂解，潜在地充当肿瘤细胞的保护因子，并作为肿瘤细胞的标记物。神经母细胞瘤细胞的检测。此外，CD276/B7-H3 可能参与急性和慢性移植排斥的发生，有助于调节粘膜表面的淋巴细胞活性。值得注意的是，它在整个怀孕期间为胎盘和胎儿提供适宜的免疫环境方面发挥着至关重要的作用。CD276/B7-H3 的亚型 1 和亚型 2 在调节 CD4 T 细胞反应的能力上似乎都是多余的，亚型 2 被证明可以增强细胞毒性 T 细胞的诱导，并在 T 存在的情况下选择性刺激干扰素 γ 的产生。-细胞受体信号传导。与 TREML2 的相互作用被确定为增强 T 细胞激活，突显了 CD276/B7-H3 在免疫调节和细胞反应中可能发挥的不同作用。

Measured by its binding ability in a functional ELISA. When immobilized Cynomolgus CD276/B7-H3- Fc at 2 μg/mL (100 μl/well), can bind Anti-Human B7-H3 with the ED₅₀ < 33.31 ng/mL.

Cynomolgus

HEK293

C-hFc

XP_015308534.1 (L29-E465)

102131295  [NCBI]

LEVQVPEDPVVALVGTDATLRCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFTEGRDQGSAYANRTALFLDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGAPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSITITPQRSPTGAVEVQVPEDPVVALVGTDATLRCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFTEGRDQGSAYANRTALFLDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGAPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMTFPPE

100-123 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.