CD28 蛋白, Human/Cynomolgus/Rhesus Macaque (HEK293, hFc)

CD28 Protein, Human/Cynomolgus/Rhesus Macaque (HEK293, hFc) is a polypeptide chain with the C-terminal human IgG1 Fc fragment produced in HEK293 cells. CD28, a T cell costimulatory receptor, is a primary target for PD-1-mediated inhibition.

CD28 是在 CD4⁺ 和 CD8⁺ T 细胞上表达的典型共刺激分子。在慢性感染和正常衰老过程中，CD28 表达丢失，损害 T 细胞的功能活性。复制应激会促进 CD28 丢失，特别是在肿瘤坏死因子-α 存在的情况下，由于 CD28 起始元件失效。与 T 细胞受体 (TCR) 相比，辅助受体 CD28 更适合作为 PD-1 招募的 Shp2 磷酸酶^[1]去磷酸化的靶标。

1.2 µg/mL (100 µL/well) of immoblized recombinant human CD28-Fc can bind human Biotin-B7-1(CD80)-Fc with a linear range of 0.01-0.5 μg/mL.
2.Immobilized Anti-CD28 mAb at 2 μg/mL (100 μl/well) can bind Human/Cynomolgus CD28-Fc *: Biotinylated by NHS-biotin prior to testing.The ED₅₀ of Human/Cynomolgus CD28-Fc * is 13.97 ng/mL.

Human

HEK293

C-hFc

P10747-1/Q0PDN3/Q0PDN4 (N19-P152)

940  [NCBI]/705313

NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP

66-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hui E, et al. T cell costimulatory receptor CD28 is a primary target for PD-1-mediated inhibition. Science. 2017 Mar 31;355(6332):1428-1433.  [Content Brief]