CD3 epsilon 蛋白, Human (27a.a, HEK293, Fc)

CD3 epsilon is an important component of the TCR-CD3 complex on T lymphocytes and promotes TCR-mediated signaling. When APC activates the TCR, CD3 epsilon, together with CD3D, CD3G, and CD3Z, transmits signals across the cell membrane through ITAMs. CD3 epsilon Protein, Human (27a.a, HEK293, Fc) is the recombinant human-derived CD3 epsilon protein, expressed by HEK293 , with C-mFc labeled tag. The total length of CD3 epsilon Protein, Human (27a.a, HEK293, Fc) is 27 a.a., with molecular weight of 32-40 kDa.

CD3 epsilon 是 T 淋巴细胞表面 TCR-CD3 复合物的组成部分，在适应性免疫反应中发挥着至关重要的作用。当抗原呈递细胞 (APC) 激活 T 细胞受体 (TCR) 时，CD3 epsilon 与其他 CD3 链（CD3D、CD3G 和 CD3Z）一起促进 TCR 介导的信号跨细胞膜的传输。CD3 epsilon 的胞质结构域中含有基于免疫受体酪氨酸的激活基序 (ITAM)，在 TCR 参与后会被 Src 家族蛋白酪氨酸激酶 LCK 和 FYN 磷酸化，从而激活下游信号通路。除了在信号转导中的作用之外，CD3 epsilon 对于 T 细胞的正常发育也至关重要，它通过形成异二聚体 CD3D/CD3E 和 CD3G/CD3E 来启动 TCR-CD3 复合物的组装。此外，CD3 epsilon 通过其胞质区域中存在的内吞序列参与 TCR-CD3 复合物的内化和细胞表面下调。TCR-CD3复合物包含CD3D/CD3E和CD3G/CD3E异二聚体，其优先与TCRα和TCRβ缔合，形成与CD3Z同二聚体相互作用的三聚体以完成六聚体TCR-CD3复合物。或者，TCRgamma 和TCRdelta 可以替代TCRalpha 和TCRbeta。CD3 epsilon 与 CD6、NCK1 和 NUMB 的相互作用进一步凸显了其在协调 T 细胞激活、发育和内化过程中的关键作用。

10 μg/mL (100 μL/well) of immobilized Human CD3E-mFc can bind Human Anti-CD3 with an ED50 value of 9.4 ng/mL, corresponding to an affinity constant of <0.001 nM.

Human

HEK293

C-mFc

P07766 (Q22-T48)

916  [NCBI]

QDGNEEMGGITQTPYKVSISGTTVILT

32-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.