CD31/PECAM-1 蛋白, Mouse (HEK293, His)

The CD31/PECAM-1 protein is a cell adhesion molecule critical for leukocyte transendothelial migration (TEM) under inflammatory conditions.CD31/PECAM-1 also regulates bradykinin receptor BDKRB2 activation and modulates ERK1/2 activation in endothelial cells.CD31/PECAM-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD31/PECAM-1 protein, expressed by HEK293 , with C-6*His labeled tag.

CD31/PECAM-1 蛋白是一种关键的细胞粘附分子，对于大多数炎症条件下的白细胞跨内皮迁移 (TEM) 至关重要。Tyr-679 在 TEM 中的关键作用强调了它需要 PECAM1 往返于侧缘回收区室 (LBRC) 的有效运输，这对于靶向迁移白细胞周围的 LBRC 膜至关重要。跨同性相互作用可能通过细胞连接促进内皮细胞与细胞的粘附，而与 CD177 的异嗜性相互作用在中性粒细胞的跨内皮迁移中发挥作用。PECAM1 的同种连接具有双重目的，通过传递分离信号来防止巨噬细胞介导的邻近活白细胞的吞噬作用，同时通过将凋亡白细胞束缚到吞噬细胞来促进巨噬细胞介导的对凋亡白细胞的吞噬作用。值得注意的是，PECAM1 调节缓激肽受体 BDKRB2 激活，并调节内皮细胞中缓激肽和高渗休克诱导的 ERK1/2 激活。它与各种伙伴（包括 BDKRB2、GNAQ、PTPN11、FER 和 CD177）的相互作用，进一步阐明了控制其不同细胞功能的复杂分子机制，而其反式同二聚化对于有效的细胞间相互作用至关重要。相互作用的细微差别，例如 Ca(2+) 依赖性和直接性，增加了 CD31/PECAM-1 调节作用的复杂性。

Mouse

HEK293

C-6*His

Q08481 (E18-K590)

18613  [NCBI]

EENSFTINSIHMESLPSWEVMNGQQLTLECLVDISTTSKSRSQHRVLFYKDDAMVYNVTSREHTESYVIPQARVFHSGKYKCTVMLNNKEKTTIEYEVKVHGVSKPKVTLDKKEVTEGGVVTVNCSLQEEKPPIFFKIEKLEVGTKFVKRRIDKTSNENFVLMEFPIEAQDHVLVFRCQAGILSGFKLQESEPIRSEYVTVQESFSTPKFEIKPPGMIIEGDQLHIRCIVQVTHLVQEFTEIIIQKDKAIVATSKQSSEAVYSVMAMVEYSGHYTCKVESNRISKASSIMVNITELFPKPKLEFSSSRLDQGELLDLSCSVSGTPVANFTIQKEETVLSQYQNFSKIAEESDSGEYSCTAGIGKVVKRSGLVPIQVCEMLSKPSIFHDAKSEIIKGHAIGISCQSENGTAPITYHLMKAKSDFQTLEVTSNDPATFTDKPTRDMEYQCRADNCHSHPAVFSEILRVRVIAPVDEVVISILSSNEVQSGSEMVLRCSVKEGTSPITFQFYKEKEDRPFHQAVVNDTQAFWHNKQASKKQEGQYYCTASNRASSMRTSPRSSTLAVRVFLAPWKK

approximately 86-106 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 5 mM EDTA, pH 7.4 or 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.