2. 重组蛋白
  3. CD Antigens
  4. B Cell CD Proteins NK Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Stem Cell CD Proteins Platelet CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  5. CD34
  6. CD34 蛋白, Mouse (HEK293, His)

CD34 蛋白, Mouse (HEK293, His)

目录号: HY-P74305
产品使用指南

CD34 蛋白作为潜在的粘附分子，通过促进干细胞附着到骨髓细胞外基质或基质细胞来帮助早期造血。它充当谱系特异性聚糖的支架，使干细胞能够与基质细胞或其他骨髓成分上的凝集素结合。此外，CD34 可以将碳水化合物配体呈递给选择素，从而促进细胞粘附过程。CD34 蛋白, Mouse (HEK293, His) 是重组的 CD34 蛋白，由 HEK293 表达，带有 C-His 标签。

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描述

CD34 protein functions as a potential adhesion molecule, aiding early hematopoiesis by facilitating stem cell attachment to the bone marrow extracellular matrix or stromal cells. It acts as a scaffold for lineage-specific glycans, enabling stem cells to bind to lectins on stromal cells or other marrow components. Moreover, CD34 may present carbohydrate ligands to selectins, contributing to cell adhesion processes. CD34 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD34 protein, expressed by HEK293 , with C-His labeled tag.
研究背景

CD34蛋白是一种可能参与早期造血作用的粘附分子，促进干细胞粘附到骨髓细胞外基质或直接粘附到基质细胞。建议其作为谱系特异性聚糖附着的支架，使干细胞能够与基质细胞或其他骨髓成分表达的凝集素结合。此外，CD34 可能在将碳水化合物配体呈递给选择素方面发挥作用，从而促进细胞粘附过程。
种属

Mouse
表达系统

HEK293
标签

C-His
蛋白编号

Q64314 (T35-T287)
基因 ID
蛋白结构
N-term
CD34 (T35-T287)
Accession # Q64314
His
C-term
同用名
Hematopoietic progenitor cell antigen CD34; CD34; HPCA1
分子量

50-60 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CD34 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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