CD38 蛋白, Human (HEK293, C-His)

The CD38 protein plays a key role in cell signaling, proficiently synthesizing cyclic ADP-ribose (cADPR) as a critical second messenger for glucose-induced insulin secretion. It also promotes the synthesis of the calcium mobilizer niacin-adenine dinucleotide phosphate (NAADP+). CD38 Protein, Human (HEK293, C-His) is the recombinant human-derived CD38 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD38 Protein, Human (HEK293, C-His) is 258 a.a., with molecular weight of 38-50 kDa.

CD38 蛋白在细胞信号传导中发挥着关键作用，是环状 ADP-核糖 (cADPR) 的熟练合成者，而 cADPR 是公认的对葡萄糖诱导的胰岛素分泌至关重要的第二信使。此外，它还促进钙动员剂烟酸-腺嘌呤二核苷酸磷酸、NAADP(+) 的合成，NAADP(+) 源自 2'-磷酸-cADPR 和烟酸，以及 NADP(+) 和烟酸。在 pH 5.0 和 pH 7.4 下工作时，CD38 蛋白优先将 2'-磷酸-cADPR 转化为 NAADP(+)，同时选择性地将 NADP(+) 裂解为 cADPR 和 ADPRP，而不是生成 NADDP(+)。值得注意的是，它还展示了 cADPR 水解酶活性，突出了其在这些关键信号分子动态调节中的多方面作用。这些活性的整合强调了 CD38 蛋白在调节细胞反应中的重要性，特别是在胰岛素分泌和钙动员的背景下。

Human

HEK293

C-6*His

P28907 (V43-I300)

952  [NCBI]

VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI

38-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 40 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.