CD3D-CD3E Heterodimer 蛋白, Cynomolgus (HEK293, Fc-Flag&Fc-His)

CD3 δ protein is a component of the TCR-CD3 complex on T lymphocytes and plays a crucial role in adaptive immunity. Activated by APC, TCR signals through the CD3 chain, including CD3D, CD3E, CD3G, and CD3Z. CD3D-CD3E Heterodimer Protein, Cynomolgus (HEK293, Fc-Flag&Fc-His) is a recombinant protein dimer complex containing cynomolgus-derived CD3D-CD3E Heterodimer protein, expressed by HEK293 , with C-hFc, C-Flag, C-6*His labeled tag. CD3D-CD3E Heterodimer Protein, Cynomolgus (HEK293, Fc-Flag&Fc-His), has molecular weight of 40-60 kDa.

CD3 δ 蛋白是 T 淋巴细胞表面 TCR-CD3 复合物的组成部分，对于适应性免疫反应至关重要。被抗原呈递细胞 (APC) 激活后，T 细胞受体 (TCR) 通过 CD3 链传递信号，包括 CD3D、CD3E、CD3G 和 CD3Z，每个链的胞质结构域均包含基于免疫受体酪氨酸的激活基序 (ITAM) 。当 TCR 参与时，这些基序会被 Src 家族蛋白酪氨酸激酶 LCK 和 FYN 磷酸化，启动下游信号传导通路。除了在 T 细胞激活的信号转导中发挥作用外，CD3D 对于胸腺细胞分化也是不可或缺的，有助于 TCR-CD3 复合物的准确组装和表面表达。在缺乏功能性 TCR-CD3 复合物的情况下，胸腺细胞难以正常分化。CD3D 与 CD4 和 CD8 相互作用，在 TCR 与辅助受体 CD4 和 CD8 之间建立功能联系，这对于 CD4 或 CD8 T 细胞的激活和正选择至关重要。TCR-CD3复合物由CD3D/CD3E和CD3G/CD3E异二聚体组成，形成TCRα/CD3E/CD3G和TCRbeta/CD3G/CD3E三聚体。该六聚体与 CD3Z 同二聚体相互作用，形成完整的 TCR-CD3 复合物。或者，TCRα和TCRβ可以被TCRγ和TCRδ替代。CD3D 复杂的相互作用强调了它在协调 T 细胞反应中的关键作用。

Cynomolgus

HEK293

C-hFc;C-Flag;C-6*His

Q95LI8 (F22-A105)&Q95LI5 (Q22-D117)

102133701  [NCBI]&102133065  [NCBI]

FKIPVEELEDRVFVKCNTSVTWVEGTVGTLLTNNTRLDLGKRILDPRGIYRCNGTDIYKDKESAVQVHYRMCQNCVELDPATLA&QDGNEEMGSITQTPYQVSISGTTVILTCSQHLGSEAQWQHNGKNKEDSGDRLFLPEFSEMEQSGYYVCYPRGSNPEDASHHLYLKARVCENCMEMD

40-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.