1. 重组蛋白
  2. CD Antigens Complement System
  3. Platelet CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Complement Regulatory Proteins
  4. Membrane Cofactor Protein/CD46
  5. CD46 蛋白, Cynomolgus (HEK293, His)

CD46 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P700681
产品使用指南 技术支持

CD46 蛋白是补体因子 I 的重要辅助因子,补体因子 I 是一种丝氨酸蛋白酶,通过裂解沉积的 C3b 和 C4b 来保护自体细胞。除了补体调节之外,CD46 还参与受精过程中的精子-卵母细胞融合。作为 T 细胞的共刺激因子,它促进 CD4+ 细胞分化为 T 调节性 1 细胞,这对于通过分泌白细胞介素 10、抑制免疫反应和预防自身免疫来进行免疫调节至关重要。CD46 蛋白, Cynomolgus (HEK293, His) 是重组的 CD46 蛋白,由 HEK293 表达,带有 C-His 标签。CD46 蛋白, Cynomolgus (HEK293, His) 全长 295 个氨基酸,分子量为 50-70 kDa。

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描述

CD46 protein acts as a crucial cofactor for complement factor I, a serine protease protecting autologous cells by cleaving deposited C3b and C4b. In addition to complement regulation, CD46 is involved in sperm-oocyte fusion during fertilization. Acting as a costimulatory factor for T-cells, it promotes the differentiation of CD4+ cells into T-regulatory 1 cells, crucial for immune modulation by secreting interleukin-10, suppressing immune responses, and preventing autoimmunity. CD46 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD46 protein, expressed by HEK293, with C-His labeled tag. The total length of CD46 Protein, Cynomolgus (HEK293, His) is 295 a.a., with molecular weight of 50-70 kDa.

研究背景

CD46 蛋白是补体因子 I 的重要辅助因子,补体因子 I 是一种丝氨酸蛋白酶,负责通过裂解宿主组织上沉积的 C3b 和 C4b 来保护自体细胞免受补体介导的损伤。除了在补体调节中的作用外,CD46 还参与受精过程中精子和卵母细胞之间的融合过程。此外,CD46 作为 T 细胞的共刺激因子,促进 CD4+ 细胞分化为 T 调节性 1 细胞。这些专门的 T 调节 1 细胞通过分泌白细胞介素 10 来促进免疫调节,从而在抑制免疫反应和预防自身免疫方面发挥关键作用。

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

A0A2K5WCS2 (C35-D329)

基因 ID

102146832

蛋白结构
N-term
CD46 (C35-D329)
Accession # A0A2K5WCS2
His
C-term
同用名
TLX; CD46; MCP; MIC10; AHUS2; TRA2.10
分子量

50-70 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD46 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P700681
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