CD79B 蛋白, Rat (HEK293, His)

CD79B Protein, also known as B29 or Igβ, is an essential component of the B cell receptor along with immunoglobulin and mb1 (Igα, CD79a) and is absolutely required for B cell development. Rat CD79B protein comprises 228 amino acids, and its amino acid sequence is 85 and 69% identical with the mouse and human counterparts, respectively. The B-cell antigen receptor (BCR) signaling component Igβ (CD79B) and the co-receptor CD19 act as an alternative B-cell signaling module that promotes the survival of B lymphoma and normal B cells via integrated ITAM/PI3K signaling. The loss of CD79B causes a block in N-glycan maturation and accumulation of immature proteins^[1][2][3]. CD79B Protein, Rat (HEK293, His) is the recombinant rat-derived CD79B protein, expressed by HEK293 , with C-His labeled tag. The total length of CD79B Protein, Rat (HEK293, His) is 133 a.a., with molecular weight of 25-30 kDa.

人类、小鼠和大鼠 B29 (Ig beta, CD79b) 基因在序列和组织上高度保守，并表现出严格的 B 细胞特异性表达。在人类和大鼠基因组中，B29 基因位于骨骼肌特异性 Na 通道 α 亚基 (SCN4A) 基因和垂体特异性生长激素 (GH-N) 基因之间。大鼠 B29/Ig-β 基因长 3.1 kb，有 6 个外显子，与 Na 通道和 GH 基因分别相隔 3.3 和 9.3 kb^[1][2]。
B 细胞受体 (BCR) 由表面结合的免疫球蛋白 (Ig) 和包含 CD79A 和 CD79B 组成的异二聚体信号单元组成。识别同源抗原后，受体会启动 B 细胞发育和功能的重要信号^[3]。

Immobilized Human CD79B at 2 μg/mL (100 μL/well) can bind Anti-CD79B Antibody. The ED₅₀ for this effect is 0.7885 μg/mL.

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  Immobilized Human CD79B at 2 μg/mL (100 μL/well) can bind Anti-CD79B Antibody. The ED₅₀ for this effect is 0.7885μg/mL.

Rat

HEK293

C-6*His

O70153 (V26-D158)

171055  [NCBI]

VPAMTKSDQPPIFQGSPCSKIWQHPRFAAKKRSSMVKFHCHTDYSGVMTWFRQKGNQRPQELFPEDGHISQTRNGSVYTLTLQNIQYEDNGIYFCQQKCNSTEPDVTDGCGTELLVLGFSTLDQLKRRNTLKD

Approximately 25-30 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.