CD8 alpha 蛋白, Rat (HEK293, Fc)

CD8 alpha is an important immune glycoprotein that serves as a coreceptor for MHC class I:peptide complexes, linking T cells to antigens presented by APCs.It interacts with TCR and MHC class I, recruits LCK kinase, and initiates multiple signaling pathways.CD8 alpha Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD8 alpha protein, expressed by HEK293 , with C-hFc labeled tag.

CD8 α 蛋白是一种完整的膜糖蛋白，在免疫反应中发挥着关键作用，在针对外部和内部威胁的反应中发挥多种功能。在 T 细胞中，它的主要功能是作为 MHC I 类分子：肽复合物的辅助受体，同时与 T 细胞受体 (TCR) 和抗原呈递细胞 (APC) 呈递的 MHC I 类蛋白相互作用。这种相互作用促进 Src 激酶 LCK 募集到 TCR-CD3 复合物附近。反过来，LCK 通过磷酸化不同的底物启动各种细胞内信号传导途径，最终导致淋巴因子的产生、运动、粘附和细胞毒性 T 淋巴细胞 (CTL) 的激活。这种机制使 CTL 能够识别并消除受感染的细胞和肿瘤细胞。在 NK 细胞中，细胞表面 CD8A 同二聚体的存在提供了一种生存机制，允许多个靶细胞的接合和裂解。CD8A 同二聚体分子还有助于活化淋巴细胞的存活和分化为记忆 CD8 T 细胞。CD8 α 在细胞表面与 CD8B 形成二硫键连接的异二聚体，并且在各种细胞类型（包括 NK 细胞和外周血 T 淋巴细胞）中形成同二聚体。此外，它还与 MHC I 类 HLA-A/B2M 二聚体相互作用，并以锌依赖性方式与 LCK 结合。

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 2.584 μg/mL, corresponding to a specific activity is 387.0 units/mg.

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  Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED₅₀ for this effect is 2.584 μg/mL, corresponding to a specific activity is 387.0 units/mg.

Rat

HEK293

C-hFc

P07725 (Q27-Y189)

24930  [NCBI]

QLQLSPKKVDAEIGQEVKLTCEVLRDTSQGCSWLFRNSSSELLQPTFIIYVSSSRSKLNDILDPNLFSARKENNKYILTLSKFSTKNQGYYFCSITSNSVMYFSPLVPVFQKVNSIITKPVTRAPTPVPPPTGTPRPLRPEACRPGASGSVEGMGLGFACDIY

Approximately 50-56 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.