CD98 蛋白, Rat (HEK293, Fc)

CD98 Protein, a chaperone, aids in the biogenesis and trafficking of functional transporter heterodimers to the plasma membrane. It forms heterodimers with SLC7 family transporters, acting as amino acid antiporters with substrate specificity determined by the SLC7A subunit. CD98 is crucial for plasma membrane localization, stability, and transport activity of SLC7A5 and SLC7A8. When associated with LAPTM4B, the SLC7A5 heterodimer is recruited to lysosomes, promoting leucine uptake and mTORC1 activation. CD98 modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration, and tumor progression. Additionally, it interacts with TLCD3A/CT120, ICAM1, and beta-1 integrins. CD98 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD98 protein, expressed by HEK293, with N-hFc labeled tag. The total length of CD98 Protein, Rat (HEK293, Fc) is 428 a.a., with molecular weight of ~95 kDa.

CD98 蛋白作为伴侣，促进功能性转运蛋白异二聚体的生物发生和运输至质膜。它与各种 SLC7 家族转运蛋白形成异二聚体，包括 SLC7A5、SLC7A6、SLC7A7、SLC7A8、SLC7A10 和 SLC7A11，充当氨基酸反向转运蛋白，其底物特异性由 SLC7A 亚基决定。例如，由 SLC3A2/SLC7A6 或 SLC3A2/SLC7A7 形成的异二聚体介导二元氨基酸的摄取，而 SLC3A2/SLC7A11 异二聚体通过跨细胞质膜交换细胞外阴离子 L-胱氨酸和细胞内 L-谷氨酸而发挥逆向转运蛋白的作用。CD98 对于 SLC7A5 和 SLC7A8 的质膜定位、稳定性和转运活性至关重要。当与 LAPTM4B 结合时，SLC7A5 异二聚体被招募到溶酶体，促进亮氨酸摄取和 mTORC1 激活。CD98 还调节整合素相关信号传导，对于整合素依赖性细胞扩散、迁移和肿瘤进展至关重要。它与非糖基化轻链（SLC7A5、SLC7A6、SLC7A7、SLC7A8、SLC7A10 或 SLC7A11）形成二硫键连接的异二聚体，并与 TLCD3A/CT120 和 ICAM1 相互作用。此外，CD98 与 β-1 整合素（包括 α-2/β-1、α-3/β-1、α-5/β-1 和 α-6/β-1）组成型、特异性结合，相互作用极小与 alpha-4/beta-1。它还与 LAPTM4B 相互作用，将 SLC3A2 和 SLC7A5 募集至溶酶体以摄取亮氨酸和激活 mTORC1。

Rat

HEK293

N-hFc

Q794F9 (A100-A527)

50567  [NCBI]

APRCRELPVQRWWHKGALYRIGDLQAFVGPEARGIAGLKNHLEYLSTLKVKGLVLGPIHKNQKDEVNETDLKQIDPDLGSQEDFKDLLQSAKKKSIHIILDLTPNYKGQNAWFLPPQADIVATKMKEALSSWLQDGVDGFQVRDVGKLANASLYLAEWQNITKNFSEDRLLIAGTASSDLQQIVNILESTSDLLLTSSYLSQPVFTGEHAELLVIKYLNATGSRWCSWSVSQAGLLTSFIPAQFLRLYQLLLFTLPGTPVFSYGDELGLQAVALPGQPMEAPFMLWNESSNSQTSSPVSLNMTVKGQNEDPGSLLTQFRRLSDLRGKERSLLHGDFDALSSSSGLFSYVRHWDQNERYLVVLNFQDVGLSARVGASNLPAGISLPASANLLLSTDSTRLSREEGTSLSLENLSLNPYEGLLLQFPFVA

Approximately 95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.