1. 重组蛋白
  2. CD Antigens
  3. NK Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Cell Adhesion-related CD Proteins
  4. CD99/MIC2
  5. CD99 蛋白, Mouse (HEK293, Fc)

CD99 蛋白, Mouse (HEK293, Fc)

目录号: HY-P75366
COA 产品使用指南

CD99 蛋白促进 T 细胞粘附,并在白细胞外渗期间帮助白细胞穿过内皮基底膜。它与 PECAM1 一起发挥作用,但又独立运作。CD99 蛋白形成同二聚体并与 PILRB 相互作用。CD99 蛋白, Mouse (HEK293, Fc) 是重组的 CD99 蛋白,由 HEK293 表达,带有 C-hFc 标签。CD99 蛋白, Mouse (HEK293, Fc) 全长 110 个氨基酸,分子量为 47-55 kDa。

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规格 价格 是否有货 数量
5 μg ¥550 In-stock
10 μg ¥950 In-stock
50 μg ¥2600 In-stock
100 μg ¥3800 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CD99 Protein facilitates T-cell adhesion and aids leukocytes in crossing the endothelial basement membrane during leukocyte extravasation. It functions alongside PECAM1 but acts independently. CD99 Protein forms a homodimer and interacts with PILRB. CD99 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD99 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD99 Protein, Mouse (HEK293, Fc) is 110 a.a., with molecular weight of 47-55 kDa.

研究背景

CD99 蛋白参与 T 细胞粘附过程,并在白细胞外渗的后期发挥关键作用,帮助白细胞克服内皮基底膜。它与 PECAM1 在同一站点运行,但功能独立。CD99 蛋白形成同二聚体并与 PILRB 相互作用。

生物活性

Immobilized Mouse CD99 Protein at 5 µg/mL (100 µL/well) can bind Mouse PILR-alpha protein. The ED50 for this effect is 0.2712 μg/mL, corresponding to a specific activity is 3687.316 Unit/mg.

  • Immobilized Mouse CD99 Protein at 5 µg/mL (100 µL/well) can bind Mouse PILR-alpha protein. The ED50 for this effect is 0.2712 μg/mL, corresponding to a specific activity is 3687.316 Unit/mg.
种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

Q8VCN6 (D29-G138)

基因 ID
蛋白结构
N-term
CD99 (D29-G138)
Accession # Q8VCN6
hFc
C-term
同用名
CD99 Antigen; 12E7; E2 Antigen; Protein MIC2; T-Cell Surface Glycoprotein E2; CD99; MIC2; MIC2X; MIC2Y
氨基酸序列

DDFNLGDALEDPNMKPTPKAPTPKKPSGGFDLEDALPGGGGGGAGEKPGNRPQPDPKPPRPHGDSGGISDSDLADAAGQGGGGAGRRGSGDEGGHGGAGGAEPEGTPQG

分子量

Approximately 47-55 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD99 Protein, Mouse (HEK293, Fc)
目录号:
HY-P75366
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