1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. Cyclin-Dependent Kinases (CDKs)
  5. Cyclin Dependent Kinase 1 (CDK1)
  6. CDK1 蛋白, Human (sf9)

CDK1 蛋白, Human (sf9)

目录号: HY-P702779
产品使用指南

CDK1/CDC2-cyclin-B 复合物是细胞周期中的关键调节因子。它在细胞分裂和发育中发挥重要作用,调节多种细胞活动,包括染色体分离、核膜破裂、胞质分裂等。CDK1的活性受多种磷酸化和去磷酸化的调节,从而控制细胞分裂的进程。细胞周期和DNA修复过程。CDK1 蛋白, Human (sf9) 是重组的 CDK1 蛋白,由 Sf9 insect cells 表达,带有 tag Free 标签。CDK1 蛋白, Human (sf9) 全长 297 个氨基酸

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描述

The CDK1/CDC2-cyclin-B complex is a key regulator in the cell cycle. It plays an important role in cell division and development and regulates a variety of cellular activities, including chromosome segregation, nuclear membrane rupture, cytokinesis, etc. The activity of CDK1 is regulated by a variety of phosphorylation and dephosphorylation, thereby controlling the progression of the cell cycle and the DNA repair process. CDK1 Protein, Human (sf9) is the recombinant human-derived CDK1, expressed by Sf9 insect cells , with tag Free labeled tag. The total length of CDK1 Protein, Human (sf9) is 297 a.a.,

研究背景

CDK1/CDC2-cyclin-B 复合物是细胞周期的关键调节因子。它在间期受精卵中起着重要的控制作用,对于胚胎发育的早期阶段至关重要。在 G2 和早期有丝分裂期间,CDC25A/B/C 介导的去磷酸化会激活 CDK1/细胞周期蛋白复合物。该复合物磷酸化多种底物,引发中心体解离、高尔基体动力学、核膜破坏和染色体浓缩。染色体在中期平板上浓缩并排列后,WEE1 和 PKMYT1 介导的磷酸化关闭 CDK1 活性,从而实现姐妹染色单体分离、染色体解浓缩、核膜重塑和胞质分裂。当DNA受损时,CDK1会通过PKR/EIF2AK2和WEE1介导的磷酸化而失活,从而停止细胞周期和基因组复制并促进DNA修复。修复完成后,CDK1 通过 WIP1 依赖性信号传导重新激活,以恢复细胞周期进程。在增殖细胞中,CDK1介导的磷酸化可以抑制FOXO1相互作用并促进FOXO1核积累和转录因子活性,导致有丝分裂后神经元的细胞死亡。此外,CDK1的磷酸化还可以调节微管动力学、纺锤体形成等过程。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

P06493 (M1-M297)

基因 ID

983

蛋白结构
N-term
CDK1 (M1-M297)
Accession # P06493
C-term
同用名
Cyclin-dependent kinase 1; CDK1; p34 protein kinase; CDC2; CDC28A; CDKN1; P34CDC2
分子量

34.25 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CDK1 Protein, Human (sf9)
目录号:
HY-P702779
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