CDK4 蛋白, Human (sf9, His, Avi)

CDK4 protein, acting as a Ser/Thr kinase in the cyclin D-CDK4 complex, critically regulates the G(1)/S transition by phosphorylating and inhibiting RB family members. This activity, specifically hypophosphorylation of RB1 during early G(1) phase, releases E2F and promotes transcription of genes that drive G(1) progression. CDK4 Protein, Human (sf9, His, Avi) is the recombinant human-derived CDK4, expressed by Sf9 insect cells , with N-Avi, N-His labeled tag. The total length of CDK4 Protein, Human (sf9, His, Avi) is 303 a.a.,

CDK4 蛋白作为细胞周期蛋白 D-CDK4 (DC) 复合物中的 Ser/Thr 激酶成分，协调属于视网膜母细胞瘤 (RB) 蛋白家族成员（包括 RB1）的磷酸化和抑制。这种调节活动对于控制 G(1)/S 转变期间的细胞周期至关重要。RB1 的磷酸化会引发转录因子 E2F 从 RB/E2F 复合物中解离，促进 E2F 靶基因的后续转录，从而驱动进展通过 G(1) 期。特别值得注意的是 RB1 的低磷酸化发生在早期 G(1) 期。作为多种有丝分裂和抗有丝分裂信号的重要整合者，细胞周期蛋白 D-CDK4 复合物在细胞周期调节中发挥着核心作用。此外，CDK4 蛋白表现出以细胞周期依赖性方式磷酸化 SMAD3 的能力，从而抑制其转录活性。CDK4是细胞周期蛋白D/CDK4/CDKN1B三元复合物的重要组成部分，对于细胞周期蛋白D-CDK4复合物的核转位和活性是不可或缺的。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-Avi;N-His

P11802-1 (M1-E303)

1019

MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE

38.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 1 mM DTT, 200 mM NaCl, 5%-20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.