CDKN1B 蛋白, Human (His)

The CDKN1B protein is a key cell cycle regulator that inhibits CDK2 when bound to cyclin A, thereby inhibiting G1 phase progression. It exhibits significant inhibitory effects on cyclin E- and cyclin A-CDK2 complexes. CDKN1B Protein, Human (His) is the recombinant human-derived CDKN1B protein, expressed by E. coli , with N-6*His labeled tag. The total length of CDKN1B Protein, Human (His) is 198 a.a., with molecular weight of ~30.0 kDa.

CDKN1B 蛋白作为细胞周期进程的关键调节因子，通过多方面的相互作用和活动发挥其影响。作为一种有效的抑制剂，CDKN1B 在与细胞周期蛋白 A 结合时抑制 CDK2 的激酶活性，同时对与 SPDYA 相关的 CDK2 表现出较小的抑制作用。该蛋白与 G1 停滞密切相关，并对细胞周期蛋白 E-和细胞周期蛋白 A-CDK2 复合物表现出明显的抑制作用。值得注意的是，它与细胞周期蛋白型 D-CDK4 形成复合物，在它们的组装、稳定性和 CCND1-CDK4 复合物激活的调节中发挥着至关重要的作用。CDKN1B 的磷酸化状态和化学计量决定了它是作为细胞周期蛋白型 D-CDK4 复合物的抑制剂还是激活剂。此外，CDKN1B 参与多种相互作用，包括与 CCNE1、COPS5、NUP50、14-3-3、AKT1、LYN、CDK2、SPDYA、cyclin D、CDK4、GRB2、PIM1、SKP1、SKP2、CKS1B、UHMK1 等蛋白质的相互作用和 CDK1，突出了其在调节细胞周期动态方面的多功能性。值得注意的是，PPM1H 对 Thr-187 的去磷酸化有助于 CDKN1B 的稳定性。

Human

E. coli

N-6*His

P46527 (M1-T198)

1027  [NCBI]

MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

Approximately 30.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.