Cellular tumor antigen P53/TP53, Human (His-SUMO)

Cellular tumor antigen P53/TP53, a tumor suppressor, induces growth arrest or apoptosis across tumor types. It regulates the cell cycle by negatively controlling genes crucial for division. TP53's pro-apoptotic activity involves BAX and FAS antigen stimulation or Bcl-2 repression. It induces apoptosis through interactions with PPP1R13B/ASPP1 or TP53BP2/ASPP2, inhibited by PPP1R13L/iASPP. Coordinating with mitochondrial PPIF, TP53 activates oxidative stress-induced necrosis independent of transcription. It induces long intergenic non-coding RNA transcription, contributing to TP53-dependent repression and apoptosis. TP53's involvement in Notch signaling, DNA damage response, and circadian clock regulation adds to its complex regulatory functions. Cellular tumor antigen P53/TP53, Human (His-SUMO) is the recombinant human-derived Cellular tumor antigen P53/TP53, expressed by E. coli, with N-SUMO, N-6*His labeled tag. The total length of Cellular tumor antigen P53/TP53, Human (His-SUMO) is 393 a.a., with molecular weight of 66 kDa.

细胞肿瘤抗原 P53/TP53 在各种肿瘤类型中发挥肿瘤抑制因子的作用，根据生理环境和细胞类型表现出诱导生长停滞或凋亡的能力。TP53 参与细胞周期调节，充当反式激活剂，通过控制该过程必需的一组基因来负向调节细胞分裂。其促凋亡活性是通过刺激 BAX 和 FAS 抗原表达或抑制 Bcl-2 表达来介导的。TP53 通过与 PPP1R13B/ASPP1 或 TP53BP2/ASPP2 相互作用诱导细胞凋亡，并且该活性被 PPP1R13L/iASPP 抑制。TP53 与线粒体 PPIF 合作，参与激活氧化应激诱导的坏死，不依赖于转录。它诱导长基因间非编码 RNA 的转录，例如 lincRNA-p21 和 lincRNA-Mkln1，这有助于 TP53 依赖性转录抑制和细胞凋亡。此外，TP53 还参与 Notch 信号交叉，防止 DNA 损伤时的 CDK7 激酶活性，并通过抑制 CLOCK-BMAL1 介导的 PER2 转录激活来调节生物钟。TP53 的不同亚型在反式激活活性、细胞凋亡诱导和生长抑制方面表现出差异，显示出其调节功能的复杂性。

Human

E. coli

N-SUMO;N-6*His

P04637 (M1-D393)

7157  [NCBI]

MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD

66 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.2 μm filtered solution in PBS, 6% Trehalose, pH 7.4 or 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.