Chemerin/RARRES2 蛋白, Human (HEK293, His)

Chemerin/RARRES2 protein is an adipokine secreted by adipocytes that is activated by CMKLR1 and serves as a CMKLR2 ligand to complexly regulate adipogenesis, metabolism, and inflammation. It actively regulates adipocyte differentiation, affects lipid and glucose metabolism, and plays a role in angiogenesis. Chemerin/RARRES2 Protein, Human (HEK293, His) is the recombinant human-derived Chemerin/RARRES2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Chemerin/RARRES2 Protein, Human (HEK293, His) is 137 a.a., with molecular weight of 16-20 kDa.

Chemerin/RARRES2 蛋白是一种脂肪细胞分泌的脂肪因子，通过激活趋化因子样受体 1 (CMKLR1) 并作为 CMKLR2 的配体，复杂地调节脂肪生成、代谢和炎症。虽然它可以以比 CMKLR1 或 CMKLR2 更低的亲和力与 CC 趋化因子受体样 2 (CCRL2) 结合，但其主要作用包括正向调节脂肪细胞分化以及调节与脂质和葡萄糖代谢相关的脂肪细胞基因表达。此外，Chemerin/RARRES2 在血管生成中发挥潜在作用，血管生成是白色脂肪组织扩张的关键过程。作为一种促炎脂肪因子，它刺激促炎和促糖尿病脂肪因子的分泌，对脂肪组织代谢功能产生不利影响，并导致全身效应，例如胰岛素敏感性受损、葡萄糖和脂质代谢改变以及其他组织中的血管功能受损。该蛋白基于不同蛋白酶的酶裂解而表现出促炎和抗炎特性，作为表达 CMKLR1 的白细胞群的趋化因子，并通过抑制 TNF/TNFA 诱导的血管内皮细胞中的 VCAM1 表达发挥抗炎作用。这种双重作用表明慢性炎症、肥胖和 2 型糖尿病和心血管疾病等相关疾病之间存在潜在联系，同时在皮肤中也表现出抗菌功能。

Measured in a cell proliferation assay using HUVEC human ureteral epithelial cell. The ED₅₀ for this effect is 7.695 ng/mL, corresponding to a specific activity is 1.3×10⁵ units/mg.

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  Measured in a cell proliferation assay using HUVEC human ureteral epithelial cell. The ED₅₀ for this effect is 7.695 ng/ml, corresponding to a specific activity is 1.3×10⁵ units/mg.

Human

HEK293

C-6*His

Q99969 (E21-S157)

5919  [NCBI]

ELTEAQRRGLQVALEEFHKHPPVQWAFQETSVESAVDTPFPAGIFVRLEFKLQQTSCRKRDWKKPECKVRPNGRKRKCLACIKLGSEDKVLGRLVHCPIETQVLREAEEHQETQCLRVQRAGEDPHSFYFPGQFAFS

16-20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.