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  3. Chemotaxis inhibitory 蛋白, S. aureus (P.pastoris, His)

Chemotaxis inhibitory 蛋白, S. aureus (P.pastoris, His)

目录号: HY-P71834
产品使用指南

趋化抑制蛋白 (CIP) 战略性地对抗宿主防御机制,抑制人类中性粒细胞和单核细胞的反应,以补充过敏毒素 C5a 和 fMLP。作为分子哨兵,CIP 直接与 C5aR 和 FPR 结合,阻碍 C5a 和 fMLP 诱导的钙反应。在这种战术干预中,CIP 充当守护者,巧妙地阻止细菌吞噬作用。Chemotaxis inhibitory 蛋白, S. aureus (P.pastoris, His) 是重组的 趋化抑制蛋白,由 P. pastoris 表达,带有 N-His 标签。

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描述

Chemotaxis Inhibitory Protein (CIP) strategically counters the host defense mechanisms, inhibiting reactions of human neutrophils and monocytes to complement anaphylatoxin C5a and fMLP. As a molecular sentinel, CIP directly engages with C5aR and FPR, obstructing calcium responses induced by C5a and fMLP. In this tactical intervention, CIP acts as a guardian, finessefully thwarting bacterium phagocytosis. Chemotaxis inhibitory Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Chemotaxis inhibitory protein, expressed by P. pastoris , with N-His labeled tag.

研究背景

趋化抑制蛋白 (CIP) 战略性地引导宿主防御机制领域,特别是对抗免疫反应的初始线。其作用在于抑制人中性粒细胞和单核细胞对补体过敏毒素 C5a 和甲酰化肽(例如 N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸 (fMLP))的反应。作为分子哨兵,CIP 直接与 C5a 受体 (C5aR) 和甲酰化肽受体 (FPR) 结合,有效阻碍 C5a 和 fMLP 诱导的钙反应。在这种战术干预中,CIP充当守护者,巧妙地阻止细菌的吞噬作用。

种属

Staphylococcus aureus

表达系统

P. pastoris

标签

N-His

蛋白编号

Q6GFB3 (F29-Y149)

基因 ID

/

蛋白结构
N-term
His
Chemotaxis inhibitory (F29-Y149)
Accession # Q6GFB3
C-term
同用名
chp; SAR2036Chemotaxis inhibitory protein; CHIPS
氨基酸序列

FTFEPFPTNEEIESNKKMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTVYLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY

分子量

Approximately 16.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Chemotaxis inhibitory Protein, S. aureus (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
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C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Chemotaxis inhibitory Protein, S. aureus (P.pastoris, His)
目录号:
HY-P71834
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