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  3. CHMP4B 蛋白, Human (His, Strep)

CHMP4B 蛋白, Human (His, Strep)

目录号: HY-P702051
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CHMP4B蛋白可能是ESCRT-III的核心成分,在多泡体(MVB)的形成和内体货物蛋白的分选中发挥关键作用。它对于递送至溶酶体的膜蛋白的降解至关重要。CHMP4B 蛋白, Human (His, Strep) 是重组的 CHMP4B 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。CHMP4B 蛋白, Human (His, Strep) 全长 224 个氨基酸。

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描述

CHMP4B protein may be a core component of ESCRT-III and plays a key role in the formation of multivesicular bodies (MVBs) and sorting of endosomal cargo proteins. It is essential for the degradation of membrane proteins delivered to lysosomes. CHMP4B Protein, Human (His, Strep) is the recombinant human-derived CHMP4B protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of CHMP4B Protein, Human (His, Strep) is 224 a.a., .

研究背景

CHMP4B 蛋白可能是转运复合物 III (ESCRT-III) 所需的内体分选的核心成分,在多囊泡体 (MVB) 的形成以及内体货物蛋白分选到这些结构中发挥着关键作用。MVB 含有腔内囊泡 (ILV),对于生长因子受体、溶酶体酶和脂质等膜蛋白被递送到溶酶体时的降解至关重要。CHMP4B 是 ESCRT-III 复合物的一部分,在 ILV 释放之前从内陷膜上解离。除了参与内体分选之外,CHMP4B 与 SPAST 一起还有助于后期后期的核膜密封和有丝分裂纺锤体分解。此外,CHMP4B 在膜裂变事件中发挥作用,例如包膜病毒(包括 HIV-1)的出芽,在 HIV-1 p6 和 p9 依赖性病毒释放过程中与 PDCD6IP 相互作用。CHMP4B 的复杂功能强调了其在介导关键细胞过程中的多功能性。

种属

Human

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

Q9H444 (M1-M224)

基因 ID

128866

蛋白结构
N-term
Strep-6*His
CHMP4B (M1-M224)
Accession # Q9H444
C-term
同用名
CHMP4B; Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; CHMP4b; SNF7 homolog associated with Alix 1; SNF7-2; hSnf7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2; hVps32-2
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CHMP4B Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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