CHRDL2 蛋白, Mouse (HEK293, His)

CHRDL2 protein is related to tumor angiogenesis and may negatively regulate BMP activity by hindering the interaction between BMP and receptors. It significantly affects cartilage formation and regeneration, suggesting a role in regulating matrix accumulation of immature mesenchymal cells. CHRDL2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHRDL2 protein, expressed by HEK293 , with C-6*His labeled tag.

CHRDL2 蛋白与肿瘤血管生成有关，通过阻碍 BMP 与各自受体的相互作用，可能充当 BMP 活性的负调节因子。它对软骨形成和再生具有显着影响，表明其在调节未成熟间充质细胞基质积累速率方面发挥作用。此外，CHRDL2可能在成肌细胞和成骨细胞分化和成熟过程中发挥调节作用。值得注意的是，CHRDL2 与 BMP 家族的各个成员相互作用，包括 GDF5、INHBA、BMP2、BMP4、BMP5、BMP6 和 BMP7，表明其参与多种细胞和发育途径。

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The IC₅₀for this effect is 51.19 ng/mL in the presence of 150 ng/mL of rhBMP-4.

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  Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The IC₅₀for this effect is 51.19 ng/mL in the presence of 150 ng/mL of rhBMP-4.

Mouse

HEK293

C-6*His

Q8VEA6-1 (Q24-L426)

69121  [NCBI]

QARSRSGKVCLFGEKIYTPGQSWHPYLEPQGTIYCVRCTCSENGHVNCYRLRCPPLHCSQPVMEPQQCCPRCVDPHVPSGLRVPLKSCQLNETTYQHGEIFSAQELFPARLSNQCVLCSCIEGHTYCGLMTCPEPSCPTTLPLPDSCCQTCKDRTTESSTEENLTQLQHGERHSQDPCSERRGPSTPAPTSLSSPLGFIPRHFQSVGMGSTTIKIILKEKHKKACTHNGKTYSHGEVWHPTVLSFGPMPCILCTCIDGYQDCHRVTCPTQYPCSQPKKVAGKCCKICPEDEAEDDHSEVISTRCPKVPGQFHVYTLASPSPDSLHRFVLEHEASDQVEMYIWKLVKGIYHLVQIKRVRKQDFQKEAQNFRLLTGTHEGYWTVFLAQTPELKVTASPDKVTKTL

57-73 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.