2. 重组蛋白
  3. Receptor Proteins
  4. CHRNA3 蛋白, Human (His-SUMO)

CHRNA3 蛋白, Human (His-SUMO)

目录号: HY-P72144
产品使用指南

CHRNA3 蛋白是胶原纤维形成的关键因素，可调节纤维形成的速率并影响胶原纤维的组装和组织。CHRNA3 与 I 型和 II 型胶原蛋白结合，在塑造细胞外基质的结构完整性方面发挥着关键作用。CHRNA3 蛋白, Human (His-SUMO) 是重组的 CHRNA3 蛋白，由 E. coli 表达，带有 N-10*His, N-SUMO 标签。CHRNA3 蛋白, Human (His-SUMO) 全长 209 个氨基酸，分子量约为 ~44.6 kDa。

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描述

CHRNA3 Protein, a critical contributor to collagen fibrillogenesis, regulates the rate of fibril formation and influences the assembly and organization of collagen fibrils. Binding to both type I and type II collagen, CHRNA3 plays a pivotal role in shaping the structural integrity of the extracellular matrix. CHRNA3 Protein, Human (His-SUMO) is the recombinant human-derived CHRNA3 protein, expressed by E. coli , with N-10*His, N-SUMO labeled tag. The total length of CHRNA3 Protein, Human (His-SUMO) is 209 a.a., with molecular weight of ~44.6 kDa.
研究背景

纤维调节蛋白是一种在胶原纤维形成中起关键作用的蛋白质，显着影响纤维形成的速率，并可能是这一过程的主要贡献者。已知纤维调节蛋白可结合 I 型和 II 型胶原蛋白，在调节胶原纤维的组装和组织方面发挥着至关重要的作用。它对原纤维生成动力学的影响表明它参与塑造细胞外基质的结构完整性。
种属

Human
表达系统

E. coli
标签

N-10*His;N-SUMO
蛋白编号

P32297 (S32-L240)
基因 ID
蛋白结构
N-term
10*His-SUMO
CHRNA3 (S32-L240)
Accession # P32297
C-term
同用名
ACHA3_HUMAN; AChR; Cholinergic receptor neuronal nicotinic alpha polypeptide 3; Cholinergic receptor nicotinic alpha 3; Cholinergic receptor nicotinic alpha polypeptide 3; CHRNA 3; CHRNA3; LNCR2; MGC104879; NACHRA 3; NACHRA3; Neuronal acetylcholine receptor protein alpha 3 chain precursor; Neuronal acetylcholine receptor subunit alpha 3; Neuronal acetylcholine receptor subunit alpha-3; Neuronal nicotinic acetylcholine receptor alpha 3 subunit; PAOD2
氨基酸序列

SEAEHRLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRL

分子量

Approximately 44.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CHRNA3 Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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