CHST3 蛋白, Mouse (HEK293, His)

The CHST3 protein utilizes PAPS to keyly catalyze the transfer of sulfate to position 6 of GalNAc in chondroitin, the major proteoglycan in cartilage. CHST3 also sulfonates Gal residues in keratan sulfate and sialic acid LacNAc oligosaccharides with less efficiency. CHST3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHST3 protein, expressed by HEK293 , with N-His labeled tag. The total length of CHST3 Protein, Mouse (HEK293, His) is 434 a.a., with molecular weight of 75-80 KDa.

CHST3 蛋白是一种利用 3'-磷酸-5'-腺苷酸硫酸盐 (PAPS) 作为磺酸盐供体的磺基转移酶，是催化硫酸盐转移到软骨素 N-乙酰半乳糖胺 (GalNAc) 残基 6 位的关键酶。软骨中占主导地位的蛋白多糖，广泛分布于细胞表面和细胞外基质。虽然效率较低，但 CHST3 还可以磺化另一种糖胺聚糖硫酸角质素的 Gal 残基，并催化唾液酸 N-乙酰基乳糖胺 (sialyl LacNAc) 寡糖中 Gal 残基的硫酸化。除了其聚糖修饰活性之外，CHST3 还可能在维持脾脏中幼稚 T 淋巴细胞方面发挥作用。CHST3 的多功能硫酸化能力强调了其在调节关键糖胺聚糖的硫酸化模式、促进细胞外基质的结构多样性以及潜在影响特定组织中的免疫细胞动力学方面的重要性。

Measured by its ability to transfer sulfate from PAPS to chondroitin sulfate. The specific activity is 3316 pmol/min/μg that incubate at 37 ºC for 20 minutes.

Mouse

HEK293

N-10*His

O88199 (E39-T472)

53374  [NCBI]

EKENKIISRVSDKLKQIPHFVADANSTDPALLLSENASLLSLSELDSTFSHLRSRLHNLSLQLGVEPAMESQEAGAEKPSQQAGAGTRRHVLLMATTRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVFFQQRGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDHLTQFLFRRGSSRSLCEDPVCTPFVKKVFEKYHCRNRRCGPLNVTLAGEACRRKDHVALKAVRIRQLEFLQPLVEDPRLDLRVIQLVRDPRAVLASRIVAFAGKYENWKKWLSEGQDQLSEDEVQRLRGNCESIRLSAELGLRQPAWLRGRYMLVRYEDVARRPLQKAREMYSFAGIPLTPQVEDWIQKNTQATRDSSDVYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPTMHLFGYKLARDAASLTNRSISLLEERGTFWVT

Approximately 75-80 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.